9ANT

ANTENNAPEDIA HOMEODOMAIN-DNA COMPLEX

Summary for 9ANT

• Entry DOI: 10.2210/pdb9ant/pdb
• Descriptor: DNA (5'-D(*AP*GP*AP*AP*AP*GP*CP*CP*AP*TP*TP*AP*GP*AP*G)-3'), DNA (5'-D(*TP*CP*TP*CP*TP*AP*AP*TP*GP*GP*CP*TP*TP*TP*C)-3'), ANTENNAPEDIA HOMEODOMAIN, ... (5 entities in total)
• Functional Keywords: homeodomain, dna-binding protein, complex (homeodomain-dna), transcription-dna complex, transcription/dna
• Biological source: Drosophila melanogaster (fruit fly)
• Cellular location: Nucleus (Probable): P02833
• Total number of polymer chains: 6
• Total formula weight: 34593.53

Authors

Fraenkel, E.,Pabo, C.O. (deposition date: 1998-07-02, release date: 1998-10-14, Last modification date: 2024-02-14)

Primary citation

Fraenkel, E.,Pabo, C.O.
Comparison of X-ray and NMR structures for the Antennapedia homeodomain-DNA complex.
Nat.Struct.Biol., 5:692-697, 1998

PubMed Abstract: Homeodomains are one of the key families of eukaryotic DNA-binding motifs and provide an important model system for studying protein-DNA interactions. We have crystallized the Antennapedia homeodomain-DNA complex and solved this structure at 2.4 A resolution. NMR and molecular dynamics studies had implied that this homeodomain achieves specificity through an ensemble of rapidly fluctuating DNA contacts. The crystal structure is in agreement with the underlying NMR data, but our structure reveals a well-defined set of contacts and also reveals the locations and roles of water molecules at the protein-DNA interface. The synthesis of X-ray and NMR studies provides a unified, general model for homeodomain-DNA interactions.

PubMed: 9699632

Experimental method

X-RAY DIFFRACTION (2.4 Å)