9ANT

ANTENNAPEDIA HOMEODOMAIN-DNA COMPLEX

9ANT の概要

• エントリーDOI: 10.2210/pdb9ant/pdb
• 分子名称: DNA (5'-D(*AP*GP*AP*AP*AP*GP*CP*CP*AP*TP*TP*AP*GP*AP*G)-3'), DNA (5'-D(*TP*CP*TP*CP*TP*AP*AP*TP*GP*GP*CP*TP*TP*TP*C)-3'), ANTENNAPEDIA HOMEODOMAIN, ... (5 entities in total)
• 機能のキーワード: homeodomain, dna-binding protein, complex (homeodomain-dna), transcription-dna complex, transcription/dna
• 由来する生物種: Drosophila melanogaster (fruit fly)
• 細胞内の位置: Nucleus (Probable): P02833
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 34593.53

構造登録者

Fraenkel, E.,Pabo, C.O. (登録日: 1998-07-02, 公開日: 1998-10-14, 最終更新日: 2024-02-14)

主引用文献

Fraenkel, E.,Pabo, C.O.
Comparison of X-ray and NMR structures for the Antennapedia homeodomain-DNA complex.
Nat.Struct.Biol., 5:692-697, 1998

PubMed Abstract: Homeodomains are one of the key families of eukaryotic DNA-binding motifs and provide an important model system for studying protein-DNA interactions. We have crystallized the Antennapedia homeodomain-DNA complex and solved this structure at 2.4 A resolution. NMR and molecular dynamics studies had implied that this homeodomain achieves specificity through an ensemble of rapidly fluctuating DNA contacts. The crystal structure is in agreement with the underlying NMR data, but our structure reveals a well-defined set of contacts and also reveals the locations and roles of water molecules at the protein-DNA interface. The synthesis of X-ray and NMR studies provides a unified, general model for homeodomain-DNA interactions.

PubMed: 9699632

実験手法

X-RAY DIFFRACTION (2.4 Å)