9QZK

SadB of Pseudomonas aeruginosa

Summary for 9QZK

• Entry DOI: 10.2210/pdb9qzk/pdb
• Descriptor: HDOD domain-containing protein (1 entity in total)
• Functional Keywords: biofilm, pseudomonas aeruginosa, structural protein
• Biological source: Pseudomonas aeruginosa
• Total number of polymer chains: 4
• Total formula weight: 201475.39

Authors

Opatowsky, Y.,Sporny, M. (deposition date: 2025-04-23, release date: 2025-05-28)

Primary citation

Ben-David, Y.,Sporny, M.,Brochin, Y.,Piscon, B.,Roth, S.,Zander, I.,Nisani, M.,Shoshani, S.,Yaron, O.,Karako-Lampert, S.,Lebenthal-Loinger, I.,Danielli, A.,Opatowsky, Y.,Banin, E.
SadB, a mediator of AmrZ proteolysis and biofilm development in Pseudomonas aeruginosa.
NPJ Biofilms Microbiomes, 11:77-77, 2025

PubMed Abstract: The ability of bacteria to commit to surface colonization and biofilm formation is a highly regulated process. In this study, we characterized the activity and structure of SadB, initially identified as a key regulator in the transition from reversible to irreversible surface attachment. Our results show that SadB acts as an adaptor protein that tightly regulates the master regulator AmrZ at the post-translational level. SadB directly binds to the C-terminal domain of AmrZ, leading to its rapid degradation, primarily by the Lon protease. Structural analysis suggests that SadB does not directly interact with small molecules upon signal transduction, differing from previous findings in Pseudomonas fluorescens. Instead, the SadB structure supports its role in mediating protein-protein interactions, establishing it as a major checkpoint for biofilm commitment.

PubMed: 40360526
DOI: 10.1038/s41522-025-00710-0

Experimental method

X-RAY DIFFRACTION (3.16 Å)