8ZVB

The ShosTA toxin-antitoxin system provides phage defence

8ZVB の概要

• エントリーDOI: 10.2210/pdb8zvb/pdb
• 分子名称: ShosT, GLYCEROL, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: shost, toxin, immune system
• 由来する生物種: Escherichia coli O1:K1 / APEC
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 49224.16

構造登録者

Yu, Y.,Chen, Q. (登録日: 2024-06-11, 公開日: 2025-04-09, 最終更新日: 2025-04-16)

主引用文献

Pu, H.,Chen, Y.,Zhao, X.,Dai, L.,Tong, A.,Tang, D.,Chen, Q.,Yu, Y.
A toxin-antitoxin system provides phage defense via DNA damage and repair.
Nat Commun, 16:3141-3141, 2025

PubMed Abstract: Widespread in bacteria and archaea, toxin-antitoxin (TA) systems have been recently demonstrated to function in phage defense. Here we characterize the anti-phage function of a type IV TA system, ShosTA. Using structural and biochemical approaches, we show that ShosT couples phosphoribosyltransferase and pyrophosphatase activities to disrupt purine metabolism, resulting in DNA duplication, cell filamentation and ultimate cell death. ShosA binds DNA and likely recruits other proteins to facilitate DNA homologous recombination to antagonize ShosT's toxicity. We identify Gp0.7 of T7 phage as a trigger for ShosTA system via shutting off the protein synthesis, and the C-terminus-mediated intrinsic instability of ShosA releases the toxicity of the existing ShosT proteins. Collectively, our results provide a novel toxin-antitoxin mechanism for anti-phage immunity and shed light on the triggering of this TA system.

PubMed: 40169592
DOI: 10.1038/s41467-025-58540-9

実験手法

X-RAY DIFFRACTION (2.04 Å)