8ZR2

Cocrystallization of engineered streptavidin with C9 oligo DNA

8ZR2 の概要

• エントリーDOI: 10.2210/pdb8zr2/pdb
• 分子名称: Streptavidin (2 entities in total)
• 機能のキーワード: biotin-binding protein, unknown function
• 由来する生物種: Streptomyces avidinii
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 56749.92

構造登録者

Minamihata, K.,Adachi, M. (登録日: 2024-06-03, 公開日: 2025-06-04)

主引用文献

Nagatani, A.,Minamihata, K.,Adachi, M.,Wakabayashi, R.,Goto, M.,Kamiya, N.
Dual-functional co-crystal of streptavidin and ssDNA: electrostatic assembly with positively charged peptide tags.
Rsc Adv, 15:6817-6822, 2025

PubMed Abstract: We have achieved a novel co-crystal in which the dual functions of the protein and single-stranded DNA are maintained by introducing a charged peptide tag at the C-terminus of the protein. The functionalities allowed the co-crystals to be modified with high selectivity. Additionally, we have confirmed that energy transfer occurs between the two molecules modified within the co-crystal. Therefore, this co-crystal has the potential as a novel biomaterial applicable to biosensors.

PubMed: 40035016
DOI: 10.1039/d4ra08326a

実験手法

X-RAY DIFFRACTION (2.5 Å)