8ZAP

ExoC110T class 1 channelrhodopsin

8ZAP の概要

• エントリーDOI: 10.2210/pdb8zap/pdb
• EMDBエントリー: 39883
• 分子名称: C110T-class1, POTASSIUM ION, RETINAL, ... (4 entities in total)
• 機能のキーワード: channelrhodopsin, membrane protein
• 由来する生物種: Hyphochytrium catenoides
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 91356.49

構造登録者

Zhang, M.F. (登録日: 2024-04-25, 公開日: 2024-09-04, 最終更新日: 2024-10-23)

主引用文献

Shan, Y.,Zhao, L.,Chen, M.,Li, X.,Zhang, M.,Pei, D.
Channelrhodopsins with distinct chromophores and binding patterns.
Nat Commun, 15:7292-, 2024

PubMed Abstract: Channelrhodopsins are popular optogenetic tools in neuroscience, but remain poorly understood mechanistically. Here we report the cryo-EM structures of channelrhodopsin-2 (ChR2) from Chlamydomonas reinhardtii and H. catenoides kalium channelrhodopsin (KCR1). We show that ChR2 recruits an endogenous N-retinylidene-PE-like molecule to a previously unidentified lateral retinal binding pocket, exhibiting a reduced light response in HEK293 cells. In contrast, H. catenoides kalium channelrhodopsin (KCR1) binds an endogenous retinal in its canonical retinal binding pocket under identical condition. However, exogenous ATR reduces the photocurrent magnitude of wild type KCR1 and also inhibits its leaky mutant C110T. Our results uncover diverse retinal chromophores with distinct binding patterns for channelrhodopsins in mammalian cells, which may further inspire next generation optogenetics for complex tasks such as cell fate control.

PubMed: 39181878
DOI: 10.1073/pnas.1818707116

実験手法

ELECTRON MICROSCOPY (2.6 Å)