8Z9A

Cryo-EM structure of the insect olfactory receptor OR5-Orco heterocomplex from Acyrthosiphon pisum bound with geranyl acetate

8Z9A の概要

• エントリーDOI: 10.2210/pdb8z9a/pdb
• EMDBエントリー: 39858
• 分子名称: Odorant receptor, ApisOrco, Odorant receptor, ApisOR5, 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE, ... (4 entities in total)
• 機能のキーワード: membrane protein
• 由来する生物種: Acyrthosiphon pisum (pea aphid); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 206931.67

構造登録者

Wang, Y.D.,Qiu, L.,Guan, Z.Y.,Wang, Q.,Wang, G.R.,Yin, P. (登録日: 2024-04-23, 公開日: 2024-06-19, 最終更新日: 2024-07-10)

主引用文献

Wang, Y.,Qiu, L.,Wang, B.,Guan, Z.,Dong, Z.,Zhang, J.,Cao, S.,Yang, L.,Wang, B.,Gong, Z.,Zhang, L.,Ma, W.,Liu, Z.,Zhang, D.,Wang, G.,Yin, P.
Structural basis for odorant recognition of the insect odorant receptor OR-Orco heterocomplex.
Science, 384:1453-1460, 2024

PubMed Abstract: Insects detect and discriminate a diverse array of chemicals using odorant receptors (ORs), which are ligand-gated ion channels comprising a divergent odorant-sensing OR and a conserved odorant receptor co-receptor (Orco). In this work, we report structures of the OR5-Orco heterocomplex from the pea aphid alone and bound to its known activating ligand, geranyl acetate. In these structures, three Orco subunits serve as scaffold components that cannot bind the ligand and remain relatively unchanged. Upon ligand binding, the pore-forming helix S7b of OR5 shifts outward from the central pore axis, causing an asymmetrical pore opening for ion influx. Our study provides insights into odorant recognition and channel gating of the OR-Orco heterocomplex and offers structural resources to support development of innovative insecticides and repellents for pest control.

PubMed: 38870272
DOI: 10.1126/science.adn6881

実験手法

ELECTRON MICROSCOPY (3 Å)