8Z2E

Crystal structure of nanobody Tnb04-1 with antibody 1F11 fab and SARS-CoV-2 RBD

8Z2E の概要

• エントリーDOI: 10.2210/pdb8z2e/pdb
• 分子名称: Tnb04-1, Spike protein S1, 1F11-H, ... (6 entities in total)
• 機能のキーワード: sars-cov-2, neutralizing nanobody, antibody, conserved epitope, viral protein/immune system, viral protein-immune system complex
• 由来する生物種: Vicugna pacos; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 83810.55

構造登録者

Wang, X.Q.,Zhang, L.Q.,Chen, J.,Dong, H.D. (登録日: 2024-04-12, 公開日: 2025-04-23, 最終更新日: 2025-11-05)

主引用文献

Dong, H.,Zhou, R.,Chen, J.,Wei, J.,Wei, Z.,Yang, Z.,Zhu, K.,Yang, Y.,Yang, Q.,Liu, N.,Chen, Y.,Wu, Y.,Liang, Y.,Zeng, Y.,Guo, Q.,Li, M.,Shan, S.,Wang, H.,Niu, M.,Yunfei Zeng, I.,Shi, X.,Zhang, Q.,Wang, X.,Chen, Z.,Zhang, L.
Super broad and protective nanobodies against Sarbecoviruses including SARS-CoV-1 and the divergent SARS-CoV-2 subvariant KP.3.1.1.
Plos Pathog., 20:e1012625-e1012625, 2024

PubMed Abstract: The ongoing evolution and immune escape of SARS-CoV-2, alongside the potential threat of SARS-CoV-1 and other sarbecoviruses, underscore the urgent need for effective strategies against their infection and transmission. This study highlights the discovery of nanobodies from immunized alpacas, which demonstrate exceptionally broad and potent neutralizing capabilities against the recently emerged and more divergent SARS-CoV-2 Omicron subvariants including JD.1.1, JN.1, KP.3, KP.3.1.1, as well as SARS-CoV-1 and coronaviruses from bats and pangolins utilizing receptor ACE2. Among these, Tnb04-1 emerges as the most broad and potent, binding to a conserved hydrophobic pocket in the spike's receptor-binding domain, distinct from the ACE2 binding site. This interaction disrupts the formation of a proteinase K-resistant core, crucial for viral-cell fusion. Notably, intranasal administration of Tnb04-1 in Syrian hamsters effectively prevented respiratory infection and transmission of the authentic Omicron XBB.1.5 subvariant. Thus, Thb04-1 holds promise in combating respiratory acquisition and transmission of diverse sarbecoviruses.

PubMed: 39527594
DOI: 10.1371/journal.ppat.1012625

実験手法

X-RAY DIFFRACTION (2.3 Å)