8YYX

Cryo-EM structure of OXGR1 bound to leukotriene E4 and Gq proteins

これはPDB形式変換不可エントリーです。

8YYX の概要

• エントリーDOI: 10.2210/pdb8yyx/pdb
• EMDBエントリー: 39682
• 分子名称: scFV16, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein G(q) subunit alpha, ... (8 entities in total)
• 機能のキーワード: gpcr, oxgr, membrane protein
• 由来する生物種: Vicugna pacos; 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 151149.67

構造登録者

Liu, A.,Liu, Y. (登録日: 2024-04-04, 公開日: 2025-04-09, 最終更新日: 2026-04-22)

主引用文献

Liu, A.,Liu, Y.,Long, Y.,Ye, R.D.
Molecular insights into ligand recognition and signaling of OXGR1.
Nat Commun, 16:11205-11205, 2025

PubMed Abstract: GPR99/OXGR1 is a G protein-coupled receptor (GPCR) with two endogenous agonists, the tricarboxylic acid cycle derivative 2-oxoglutarate (α-ketoglutarate) and the inflammatory mediator cysteinyl leukotriene E4 (LTE), hence also termed CysLT3 receptor. How GPR99/OXGR1 recognizes two distinct ligands is a biologically important question. Here we present cryo-EM structures of GPR99/OXGR1-Gq complexed with oxoglutarate and LTE, respectively. The oxoglutarate-bound structure shows a binding pocket surrounded by the transmembrane domains (TM), with a primary site and an accessory site for simultaneous binding of two oxoglutarate molecules for full activation of the receptor. The TM binding pocket, however, is too small to accommodate the cysteinyl leukotriene LTE. Alanine substitution of key residues for oxoglutarate binding had little impact on LTE-induced signaling. A distinct site in between TM3/4/5 just above intracellular loop 2 was identified in the solved structure with LTE, but the densities were less well-defined. Alanine substitution of amino acids potentially involved in LTE interaction at this site abrogated LTE-induced receptor activation without affecting oxoglutarate-induced signaling. Both ligands activated GPR99/OXGR1 primarily through the Gq pathway, but LTE also induced Gi signaling. These findings illustrate the structural basis for GPR99/OXGR1 to interact with structurally distict oxoglutarate and LTE.

PubMed: 41413034
DOI: 10.1038/s41467-025-67101-z

実験手法

ELECTRON MICROSCOPY (2.84 Å)