8YVI

Cryo-EM structure of carboxysomal midi-shell: icosahedral assembly from CsoS4A/4B/1A/1B/1C/1D and CsoS2 C-terminal co-expression (T = 13)

8YVI の概要

• エントリーDOI: 10.2210/pdb8yvi/pdb
• EMDBエントリー: 39601
• 分子名称: Major carboxysome shell protein CsoS1A, Carboxysome shell vertex protein CsoS4A, Carboxysome assembly protein CsoS2B (3 entities in total)
• 機能のキーワード: carboxysome, shell, icosahedron, structural protein
• 由来する生物種: Halothiobacillus neapolitanus; 詳細
• タンパク質・核酸の鎖数: 15
• 化学式量合計: 187298.88

構造登録者

Wang, P.,Li, J.X.,Li, T.P.,Li, K.,Ng, P.C.,Wang, S.M.,Chriscoli, V.,Basle, A.,Marles-Wright, J.,Zhang, Y.Z.,Liu, L.N. (登録日: 2024-03-28, 公開日: 2024-12-11)

主引用文献

Wang, P.,Li, J.,Li, T.,Li, K.,Ng, P.C.,Wang, S.,Chriscoli, V.,Basle, A.,Marles-Wright, J.,Zhang, Y.Z.,Liu, L.N.
Molecular principles of the assembly and construction of a carboxysome shell.
Sci Adv, 10:eadr4227-eadr4227, 2024

PubMed Abstract: Intracellular compartmentalization enhances biological reactions, crucial for cellular function and survival. An example is the carboxysome, a bacterial microcompartment for CO fixation. The carboxysome uses a polyhedral protein shell made of hexamers, pentamers, and trimers to encapsulate Rubisco, increasing CO levels near Rubisco to enhance carboxylation. Despite their role in the global carbon cycle, the molecular mechanisms behind carboxysome shell assembly remain unclear. Here, we present a structural characterization of α-carboxysome shells generated from recombinant systems, which contain all shell proteins and the scaffolding protein CsoS2. Atomic-resolution cryo-electron microscopy of the shell assemblies, with a maximal size of 54 nm, unveil diverse assembly interfaces between shell proteins, detailed interactions of CsoS2 with shell proteins to drive shell assembly, and the formation of heterohexamers and heteropentamers by different shell protein paralogs, facilitating the assembly of larger empty shells. Our findings provide mechanistic insights into the construction principles of α-carboxysome shells and the role of CsoS2 in governing α-carboxysome assembly and functionality.

PubMed: 39612341
DOI: 10.1126/sciadv.adr4227

実験手法

ELECTRON MICROSCOPY (2.93 Å)