Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

8YUU

Cryo-EM structure of the histamine-bound H3R-Gi complex

Summary for 8YUU
Entry DOI10.2210/pdb8yuu/pdb
EMDB information39583
DescriptorHistamine H3 receptor, Guanine nucleotide-binding protein G(i) subunit alpha-1, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, ... (7 entities in total)
Functional Keywordsgpcr, h3r, histamine receptor, membrane protein/immune system, membrane protein-immune system complex
Biological sourceHomo sapiens (human)
More
Total number of polymer chains5
Total formula weight167607.35
Authors
Shen, Q.,Tang, X.,Wen, X.,Cheng, S.,Xiao, P.,Zang, S.,Shen, D.,Jiang, L.,Zheng, Y.,Zhang, H.,Xu, H.,Mao, C.,Zhang, M.,Hu, W.,Sun, J.,Chen, Z.,Zhang, Y. (deposition date: 2024-03-27, release date: 2024-06-05, Last modification date: 2024-10-30)
Primary citationShen, Q.,Tang, X.,Wen, X.,Cheng, S.,Xiao, P.,Zang, S.K.,Shen, D.D.,Jiang, L.,Zheng, Y.,Zhang, H.,Xu, H.,Mao, C.,Zhang, M.,Hu, W.,Sun, J.P.,Zhang, Y.,Chen, Z.
Molecular Determinant Underlying Selective Coupling of Primary G-Protein by Class A GPCRs.
Adv Sci, 11:e2310120-e2310120, 2024
Cited by
PubMed Abstract: G-protein-coupled receptors (GPCRs) transmit downstream signals predominantly via G-protein pathways. However, the conformational basis of selective coupling of primary G-protein remains elusive. Histamine receptors HR and HR couple with G- or G-proteins respectively. Here, three cryo-EM structures of HR-G and HR-G complexes are presented at a global resolution of 2.6-2.7 Å. These structures reveal the unique binding pose for endogenous histamine in HR, wherein the amino group interacts with E206 of HR instead of the conserved D114 of other aminergic receptors. Furthermore, comparative analysis of the HR-G and HR-G complexes reveals that the structural geometry of TM5/TM6 determines the primary G-protein selectivity in histamine receptors. Machine learning (ML)-based structuromic profiling and functional analysis of class A GPCR-G-protein complexes illustrate that TM5 length, TM5 tilt, and TM6 outward movement are key determinants of the G and G selectivity among the whole Class A family. Collectively, the findings uncover the common structural geometry within class A GPCRs that determines the primary G- and G-coupling selectivity.
PubMed: 38647423
DOI: 10.1002/advs.202310120
PDB entries with the same primary citation
Experimental method
ELECTRON MICROSCOPY (2.7 Å)
Structure validation

226707

건을2024-10-30부터공개중

PDB statisticsPDBj update infoContact PDBjnumon