8YTY

The M12+P185V variant of Kubu-PETase from Kutzneria buriramensis

8YTY の概要

• エントリーDOI: 10.2210/pdb8yty/pdb
• 関連するPDBエントリー: 8YTU 8YTV 8YTW
• 分子名称: Dienelactone hydrolase (2 entities in total)
• 機能のキーワード: pet hydrolase, hydrolase
• 由来する生物種: Kutzneria buriramensis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27042.31

構造登録者

Park, J.,Seo, H.,Hong, H.,Kim, K.-J. (登録日: 2024-03-26, 公開日: 2025-01-15)

主引用文献

Seo, H.,Hong, H.,Park, J.,Lee, S.H.,Ki, D.,Ryu, A.,Sagong, H.Y.,Kim, K.J.
Landscape profiling of PET depolymerases using a natural sequence cluster framework.
Science, 387:eadp5637-eadp5637, 2025

PubMed Abstract: Enzymes capable of breaking down polymers have been identified from natural sources and developed for industrial use in plastic recycling. However, there are many potential starting points for enzyme optimization that remain unexplored. We generated a landscape of 170 lineages of 1894 polyethylene terephthalate depolymerase (PETase) candidates and performed profiling using sampling approaches with features associated with PET-degrading capabilities. We identified three promising yet unexplored PETase lineages and two potent PETases, Mipa-P and Kubu-P. An engineered variant of Kubu-P outperformed benchmarks in terms of PET depolymerization in harsh environments, such as those with high substrate load and ethylene glycol as the solvent.

PubMed: 39745946
DOI: 10.1126/science.adp5637

実験手法

X-RAY DIFFRACTION (1.15 Å)