8YTU

Mipa-PETase from Micromonospora pattaloongensis

Summary for 8YTU

• Entry DOI: 10.2210/pdb8ytu/pdb
• Descriptor: cutinase, 1,2-ETHANEDIOL, GLYCEROL, ... (4 entities in total)
• Functional Keywords: pet hydrolase, hydrolase
• Biological source: Micromonospora pattaloongensis
• Total number of polymer chains: 3
• Total formula weight: 84004.52

Authors

Hong, H.,Seo, H.,Park, J.,Kim, K.-J. (deposition date: 2024-03-26, release date: 2025-01-15)

Primary citation

Seo, H.,Hong, H.,Park, J.,Lee, S.H.,Ki, D.,Ryu, A.,Sagong, H.Y.,Kim, K.J.
Landscape profiling of PET depolymerases using a natural sequence cluster framework.
Science, 387:eadp5637-eadp5637, 2025

PubMed Abstract: Enzymes capable of breaking down polymers have been identified from natural sources and developed for industrial use in plastic recycling. However, there are many potential starting points for enzyme optimization that remain unexplored. We generated a landscape of 170 lineages of 1894 polyethylene terephthalate depolymerase (PETase) candidates and performed profiling using sampling approaches with features associated with PET-degrading capabilities. We identified three promising yet unexplored PETase lineages and two potent PETases, Mipa-P and Kubu-P. An engineered variant of Kubu-P outperformed benchmarks in terms of PET depolymerization in harsh environments, such as those with high substrate load and ethylene glycol as the solvent.

PubMed: 39745946
DOI: 10.1126/science.adp5637

Experimental method

X-RAY DIFFRACTION (1.34 Å)