8YT7
Crystal structure of the MAM domain of Spodoptera frugiperda Scavenger Receptor-C
Summary for 8YT7
| Entry DOI | 10.2210/pdb8yt7/pdb |
| Descriptor | Scavenger receptor SR-C-like protein, 2-acetamido-2-deoxy-beta-D-glucopyranose, CALCIUM ION, ... (4 entities in total) |
| Functional Keywords | scavenger receptor, mam domain, spodoptera frugiperda, membrane protein |
| Biological source | Spodoptera frugiperda (fall armyworm) |
| Total number of polymer chains | 1 |
| Total formula weight | 22429.84 |
| Authors | Lan, J.,Wang, C.H. (deposition date: 2024-03-25, release date: 2025-04-02, Last modification date: 2025-04-16) |
| Primary citation | Wang, C.,Li, M.,Chen, X.,Fan, S.,Lan, J. Binding Analysis of Sf-SR-C MAM Domain and Sf-FGFR Ectodomain to Vip3Aa. Insects, 15:-, 2024 Cited by PubMed Abstract: Vip3Aa has been widely used in transgenic crops to resist the erosion of insects. The Scavenger Receptor-C (SR-C) and Fibroblast Growth Factor Receptor (FGFR) of (Sf-SR-C and Sf-FGFR) have formerly been identified as the cell receptors of Vip3Aa. However, the interaction mechanism of Vip3Aa binding to Sf-SR-C or Sf-FGFR is still unknown. Here, we purified the MAM domain of Sf-SR-C (Sf-MAM) and the Sf-FGFR ectodomain expressed extracellularly by Sf9 cells. We then solved the crystal structure of the Sf-MAM domain. Structure docking analysis of the Sf-MAM and Vip3Aa C-terminal domain (CTD) excluded the possibility of the two proteins binding. A further surface plasmon resonance (SPR) assay also revealed that the Sf-MAM and Sf-FGFR ectodomain could not bind to the Vip3Aa protein. Our results have raised the urgency of determining the authentic cell receptor for Vip3Aa. PubMed: 38921143DOI: 10.3390/insects15060428 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.25 Å) |
Structure validation
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