8YT7

Crystal structure of the MAM domain of Spodoptera frugiperda Scavenger Receptor-C

8YT7 の概要

• エントリーDOI: 10.2210/pdb8yt7/pdb
• 分子名称: Scavenger receptor SR-C-like protein, 2-acetamido-2-deoxy-beta-D-glucopyranose, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: scavenger receptor, mam domain, spodoptera frugiperda, membrane protein
• 由来する生物種: Spodoptera frugiperda (fall armyworm)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22429.84

構造登録者

Lan, J.,Wang, C.H. (登録日: 2024-03-25, 公開日: 2025-04-02, 最終更新日: 2025-04-16)

主引用文献

Wang, C.,Li, M.,Chen, X.,Fan, S.,Lan, J.
Binding Analysis of Sf-SR-C MAM Domain and Sf-FGFR Ectodomain to Vip3Aa.
Insects, 15:-, 2024

PubMed Abstract: Vip3Aa has been widely used in transgenic crops to resist the erosion of insects. The Scavenger Receptor-C (SR-C) and Fibroblast Growth Factor Receptor (FGFR) of (Sf-SR-C and Sf-FGFR) have formerly been identified as the cell receptors of Vip3Aa. However, the interaction mechanism of Vip3Aa binding to Sf-SR-C or Sf-FGFR is still unknown. Here, we purified the MAM domain of Sf-SR-C (Sf-MAM) and the Sf-FGFR ectodomain expressed extracellularly by Sf9 cells. We then solved the crystal structure of the Sf-MAM domain. Structure docking analysis of the Sf-MAM and Vip3Aa C-terminal domain (CTD) excluded the possibility of the two proteins binding. A further surface plasmon resonance (SPR) assay also revealed that the Sf-MAM and Sf-FGFR ectodomain could not bind to the Vip3Aa protein. Our results have raised the urgency of determining the authentic cell receptor for Vip3Aa.

PubMed: 38921143
DOI: 10.3390/insects15060428

実験手法

X-RAY DIFFRACTION (2.25 Å)