8YM3

Structural basis of Elongation factor Tu in regulating photoinhibition in Synechococcus sp. PCC 7942.

8YM3 の概要

• エントリーDOI: 10.2210/pdb8ym3/pdb
• 分子名称: Elongation factor Tu, GLYCEROL, GUANOSINE-5'-DIPHOSPHATE, ... (6 entities in total)
• 機能のキーワード: elongation factor, cyanobacteria pcc 7942, photosystem 2, photoremediation, photosynthesis
• 由来する生物種: Synechococcus elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805) (Anacystis nidulans R2)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 44921.03

構造登録者

Cheng, C.,Ma, H. (登録日: 2024-03-08, 公開日: 2025-03-19, 最終更新日: 2025-10-01)

主引用文献

Cheng, C.,Lu, D.,Sun, H.,Zhang, K.,Yin, L.,Luan, G.,Liu, Y.,Ma, H.,Lu, X.
Structural insight into the functional regulation of Elongation factor Tu by reactive oxygen species in Synechococcus elongatus PCC 7942.
Int.J.Biol.Macromol., 277:133632-133632, 2024

PubMed Abstract: In cyanobacteria, Elongation factor Tu (EF-Tu) plays a crucial role in the repair of photosystem II (PSII), which is highly susceptible to oxidative stress induced by light exposure and regulated by reactive oxygen species (ROS). However, the specific molecular mechanism governing the functional regulation of EF-Tu by ROS remains unclear. Previous research has shown that a mutated EF-Tu, where C82 is substituted with a Ser residue, can alleviate photoinhibition, highlighting the important role of C82 in EF-Tu photosensitivity. In this study, we elucidated how ROS deactivate EF-Tu by examining the crystal structures of EF-Tu in both wild-type and mutated form (C82S) individually at resolutions of 1.7 Å and 2.0 Å in Synechococcus elongatus PCC 7942 complexed with GDP. Specifically, the GDP-bound form of EF-Tu adopts an open conformation with C82 located internally, making it resistant to oxidation. Coordinated conformational changes in switches I and II create a tunnel that positions C82 for ROS interaction, revealing the vulnerability of the closed conformation of EF-Tu to oxidation. An analysis of these two structures reveals that the precise spatial arrangement of C82 plays a crucial role in modulating EF-Tu's response to ROS, serving as a regulatory element that governs photosynthetic biosynthesis.

PubMed: 38971279
DOI: 10.1016/j.ijbiomac.2024.133632

実験手法

X-RAY DIFFRACTION (2.05 Å)