8YLD

State 4a (S4a) of yeast 80S ribosome bound to 2 tRNAs and open eEF3 and eEF2 during translocation

これはPDB形式変換不可エントリーです。

8YLD の概要

• エントリーDOI: 10.2210/pdb8yld/pdb
• EMDBエントリー: 38666
• 分子名称: Large ribosomal subunit protein eL18A, Large ribosomal subunit protein eL6B, Large ribosomal subunit protein uL30A, ... (81 entities in total)
• 機能のキーワード: 80s, eef3, eef2, ribosome
• 由来する生物種: Saccharomyces cerevisiae S288C; 詳細
• タンパク質・核酸の鎖数: 82
• 化学式量合計: 3277780.25

構造登録者

Cheng, J.,Wu, C.L.,Li, J.X.,Zhang, X.Z. (登録日: 2024-03-06, 公開日: 2024-12-11, 最終更新日: 2025-07-02)

主引用文献

Cheng, J.,Wu, C.,Li, J.,Yang, Q.,Zhao, M.,Zhang, X.
Capturing eukaryotic ribosome dynamics in situ at high resolution.
Nat.Struct.Mol.Biol., 32:698-708, 2025

PubMed Abstract: Many protein complexes are highly dynamic in cells; thus, characterizing their conformational changes in cells is crucial for unraveling their functions. Here, using cryo-electron microscopy, 451,700 ribosome particles from Saccharomyces cerevisiae cell lamellae were obtained to solve the 60S region to 2.9-Å resolution by in situ single-particle analysis. Over 20 distinct conformations were identified by three-dimensional classification with resolutions typically higher than 4 Å. These conformations were used to reconstruct a complete elongation cycle of eukaryotic translation with elongation factors (eEFs). We found that compact eEF2 anchors to the partially rotated ribosome after subunit rolling and hypothesize that it stabilizes the local conformation for peptidyl transfer. Moreover, open-eEF3 binding to a fully rotated ribosome was observed, whose conformational change was coupled with head swiveling and body back-rotation of the 40S subunit.

PubMed: 39789210
DOI: 10.1038/s41594-024-01454-9

実験手法

ELECTRON MICROSCOPY (3.9 Å)