8Y86

Human AE3 with NaHCO3-

8Y86 の概要

• エントリーDOI: 10.2210/pdb8y86/pdb
• EMDBエントリー: 39035
• 分子名称: Anion exchange protein 3, BICARBONATE ION (2 entities in total)
• 機能のキーワード: bicarbonate, transport protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 272045.07

構造登録者

Jian, L.,Zhang, Q.,Yao, D.,Cao, Y. (登録日: 2024-02-06, 公開日: 2024-07-31, 最終更新日: 2025-07-02)

主引用文献

Jian, L.,Zhang, Q.,Yao, D.,Wang, Q.,Chen, M.,Xia, Y.,Li, S.,Shen, Y.,Cao, M.,Qin, A.,Li, L.,Cao, Y.
The structural insight into the functional modulation of human anion exchanger 3.
Nat Commun, 15:6134-6134, 2024

PubMed Abstract: Anion exchanger 3 (AE3) is pivotal in regulating intracellular pH across excitable tissues, yet its structural intricacies and functional dynamics remain underexplored compared to other anion exchangers. This study unveils the structural insights into human AE3, including the cryo-electron microscopy structures for AE3 transmembrane domains (TMD) and a chimera combining AE3 N-terminal domain (NTD) with AE2 TMD (hAE32). Our analyzes reveal a substrate binding site, an NTD-TMD interlock mechanism, and a preference for an outward-facing conformation. Unlike AE2, which has more robust acid-loading capabilities, AE3's structure, including a less stable inward-facing conformation due to missing key NTD-TMD interactions, contributes to its moderated pH-modulating activity and increased sensitivity to the inhibitor DIDS. These structural differences underline AE3's distinct functional roles in specific tissues and underscore the complex interplay between structural dynamics and functional specificity within the anion exchanger family, enhancing our understanding of the physiological and pathological roles of the anion exchanger family.

PubMed: 39033175
DOI: 10.1038/s41467-024-50572-x

実験手法

ELECTRON MICROSCOPY (2.75 Å)