8Y81

Structure of the ige-fc bound to its high affinity receptor fc(epsilon)ri

8Y81 の概要

• エントリーDOI: 10.2210/pdb8y81/pdb
• EMDBエントリー: 39029
• 分子名称: High affinity immunoglobulin epsilon receptor subunit alpha, High affinity immunoglobulin epsilon receptor subunit beta, High affinity immunoglobulin epsilon receptor subunit gamma, ... (8 entities in total)
• 機能のキーワード: complex, antibody, immune system
• 由来する生物種: Rattus norvegicus (Norway rat); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 168317.67

構造登録者

Du, S.,Deng, M.J.,Xiao, J.Y. (登録日: 2024-02-05, 公開日: 2024-07-10, 最終更新日: 2025-07-02)

主引用文献

Deng, M.,Du, S.,Hou, H.,Xiao, J.
Structural insights into the high-affinity IgE receptor Fc epsilon RI complex.
Nature, 633:952-959, 2024

PubMed Abstract: Immunoglobulin E (IgE) plays a pivotal role in allergic responses. The high-affinity IgE receptor, FcεRI, found on mast cells and basophils, is central to the effector functions of IgE. FcεRI is a tetrameric complex, comprising FcεRIα, FcεRIβ and a homodimer of FcRγ (originally known as FcεRIγ), with FcεRIα recognizing the Fc region of IgE (Fcε) and FcεRIβ-FcRγ facilitating signal transduction. Additionally, FcRγ is a crucial component of other immunoglobulin receptors, including those for IgG (FcγRI and FcγRIIIA) and IgA (FcαRI). However, the molecular basis of FcεRI assembly and the structure of FcRγ have remained elusive. Here we elucidate the cryogenic electron microscopy structure of the Fcε-FcεRI complex. FcεRIα has an essential role in the receptor's assembly, interacting with FcεRIβ and both FcRγ subunits. FcεRIβ is structured as a compact four-helix bundle, similar to the B cell antigen CD20. The FcRγ dimer exhibits an asymmetric architecture, and coils with the transmembrane region of FcεRIα to form a three-helix bundle. A cholesterol-like molecule enhances the interaction between FcεRIβ and the FcεRIα-FcRγ complex. Our mutagenesis analyses further indicate similarities between the interaction of FcRγ with FcεRIα and FcγRIIIA, but differences in that with FcαRI. These findings deepen our understanding of the signalling mechanisms of FcεRI and offer insights into the functionality of other immune receptors dependent on FcRγ.

PubMed: 39169187
DOI: 10.1038/s41586-024-07864-5

実験手法

ELECTRON MICROSCOPY (2.89 Å)