8Y4U

Crystal structure of a His1 from oryza sativa

8Y4U の概要

• エントリーDOI: 10.2210/pdb8y4u/pdb
• 分子名称: Fe(II)/2-oxoglutarate-dependent oxygenase, FE (III) ION (3 entities in total)
• 機能のキーワード: hppd inhibitor sensitive 1; oxygenase;, plant protein
• 由来する生物種: Oryza sativa subsp. japonica (Rice)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 40224.29

構造登録者

Wang, N.,Ma, J.M.,Shibing, H.,Beibei, Y.,He, Z.,Dandan, L. (登録日: 2024-01-30, 公開日: 2024-02-28, 最終更新日: 2024-04-03)

主引用文献

Wang, N.,He, S.,Yang, B.,Zhang, H.,Liu, D.,Song, P.,Chen, T.,Wang, W.,Ge, H.,Ma, J.
Crystal structure of HPPD inhibitor sensitive protein from Oryza sativa.
Biochem.Biophys.Res.Commun., 704:149672-149672, 2024

PubMed Abstract: 4-hydroxyphenylpyruvate dioxygenase (HPPD) Inhibitor Sensitive 1 (HIS1) is an endogenous gene of rice, conferring broad-spectrum resistance to β-triketone herbicides. Similar genes, known as HIS1-like genes (HSLs), exhibit analogous functions and can complement the herbicide-resistant characteristics endowed by HIS1. The identification of HIS1 and HSLs represents a valuable asset, as the intentional pairing of herbicides with resistance genes emerges as an effective strategy for crop breeding. Encoded by HIS1 is a Fe(II)/2-oxoglutarate-dependent oxygenase responsible for detoxifying β-triketone herbicides through hydroxylation. However, the precise structure supporting this function remains unclear. This work, which determined the crystal structure of HIS1, reveals a conserved core motif of Fe(II)/2-oxoglutarate-dependent oxygenase and pinpoints the crucial residue dictating substrate preference between HIS1 and HSL.

PubMed: 38401306
DOI: 10.1016/j.bbrc.2024.149672

実験手法

X-RAY DIFFRACTION (2.4 Å)