8XI3

Structure of mouse SCMC-14-3-3gama complex

8XI3 の概要

• エントリーDOI: 10.2210/pdb8xi3/pdb
• EMDBエントリー: 38369
• 分子名称: 14-3-3 protein gamma, NACHT, LRR and PYD domains-containing protein 5, Transducin-like enhancer protein 6, ... (4 entities in total)
• 機能のキーワード: complex, oocyte subcortical, phosphorylation, 14-3-3, cytosolic protein
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 258563.47

構造登録者

Chi, P.,Han, Z.,Jiao, H.,Li, J.,Wang, X.,Hu, H.,Deng, D. (登録日: 2023-12-19, 公開日: 2024-10-02, 最終更新日: 2025-06-18)

主引用文献

Han, Z.,Wang, R.,Chi, P.,Zhang, Z.,Min, L.,Jiao, H.,Ou, G.,Zhou, D.,Qin, D.,Xu, C.,Gao, Z.,Qi, Q.,Li, J.,Lu, Y.,Wang, X.,Chen, J.,Yu, X.,Hu, H.,Li, L.,Deng, D.
The subcortical maternal complex modulates the cell cycle during early mammalian embryogenesis via 14-3-3.
Nat Commun, 15:8887-8887, 2024

PubMed Abstract: The subcortical maternal complex (SCMC) is essential for safeguarding female fertility in mammals. Assembled in oocytes, the SCMC maintains the cleavage of early embryos, but the underlying mechanism remains unclear. Here, we report that 14-3-3, a multifunctional protein, is a component of the SCMC. By resolving the structure of the 14-3-3-containing SCMC, we discover that phosphorylation of TLE6 contributes to the recruitment of 14-3-3. Mechanistically, during maternal-to-embryo transition, the SCMC stabilizes 14-3-3 protein and contributes to the proper control of CDC25B, thus ensuring the activation of the maturation-promoting factor and mitotic entry in mouse zygotes. Notably, the SCMC establishes a conserved molecular link with 14-3-3 and CDC25B in human oocytes/embryos. This study discloses the molecular mechanism through which the SCMC regulates the cell cycle in early embryos and elucidates the function of the SCMC in mammalian early embryogenesis.

PubMed: 39406751
DOI: 10.1038/s41467-024-53277-3

実験手法

ELECTRON MICROSCOPY (3 Å)