8XAV

Cryo-EM structure of an anti-phage defense complex

これはPDB形式変換不可エントリーです。

8XAV の概要

• エントリーDOI: 10.2210/pdb8xav/pdb
• EMDBエントリー: 38204
• 分子名称: ATP-binding protein, DUF4297 (2 entities in total)
• 機能のキーワード: duf4297-hera, nuclease, helicase, dna binding protein
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 18
• 化学式量合計: 952883.67

構造登録者

Wang, Y.,Deng, Z. (登録日: 2023-12-05, 公開日: 2024-06-05, 最終更新日: 2025-06-25)

主引用文献

An, Q.,Wang, Y.,Tian, Z.,Han, J.,Li, J.,Liao, F.,Yu, F.,Zhao, H.,Wen, Y.,Zhang, H.,Deng, Z.
Molecular and structural basis of an ATPase-nuclease dual-enzyme anti-phage defense complex.
Cell Res., 34:545-555, 2024

PubMed Abstract: Coupling distinct enzymatic effectors emerges as an efficient strategy for defense against phage infection in bacterial immune responses, such as the widely studied nuclease and cyclase activities in the type III CRISPR-Cas system. However, concerted enzymatic activities in other bacterial defense systems are poorly understood. Here, we biochemically and structurally characterize a two-component defense system DUF4297-HerA, demonstrating that DUF4297-HerA confers resistance against phage infection by cooperatively cleaving dsDNA and hydrolyzing ATP. DUF4297 alone forms a dimer, and HerA alone exists as a nonplanar split spiral hexamer, both of which exhibit extremely low enzymatic activity. Interestingly, DUF4297 and HerA assemble into an approximately 1 MDa supramolecular complex, where two layers of DUF4297 (6 DUF4297 molecules per layer) linked via inter-layer dimerization of neighboring DUF4297 molecules are stacked on top of the HerA hexamer. Importantly, the complex assembly promotes dimerization of DUF4297 molecules in the upper layer and enables a transition of HerA from a nonplanar hexamer to a planar hexamer, thus activating their respective enzymatic activities to abrogate phage infection. Together, our findings not only characterize a novel dual-enzyme anti-phage defense system, but also reveal a unique activation mechanism by cooperative complex assembly in bacterial immunity.

PubMed: 38834762
DOI: 10.1038/s41422-024-00981-w

実験手法

ELECTRON MICROSCOPY (2.87 Å)