8X8A

Crystal structure of STBD1 LIR motif in complex with GABARAPL1

8X8A の概要

• エントリーDOI: 10.2210/pdb8x8a/pdb
• 分子名称: Gamma-aminobutyric acid receptor-associated protein-like 1, Starch-binding domain-containing protein 1 (3 entities in total)
• 機能のキーワード: stbd1, lir, gabarapl1, protein binding
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 16094.20

構造登録者

Zhang, Y.C.,Pan, L.F. (登録日: 2023-11-27, 公開日: 2024-09-18)

主引用文献

Zhang, Y.,Sun, Y.,Shi, J.,Xu, P.,Wang, Y.,Liu, J.,Gong, X.,Wang, Y.,Tang, Y.,Liu, H.,Zhou, X.,Lin, Z.,Baba, O.,Morita, T.,Yu, B.,Pan, L.
Decoding the molecular mechanism of selective autophagy of glycogen mediated by autophagy receptor STBD1.
Proc.Natl.Acad.Sci.USA, 121:e2402817121-e2402817121, 2024

PubMed Abstract: Autophagy of glycogen (glycophagy) is crucial for the maintenance of cellular glucose homeostasis and physiology in mammals. STBD1 can serve as an autophagy receptor to mediate glycophagy by specifically recognizing glycogen and relevant key autophagic factors, but with poorly understood mechanisms. Here, we systematically characterize the interactions of STBD1 with glycogen and related saccharides, and determine the crystal structure of the STBD1 CBM20 domain with maltotetraose, uncovering a unique binding mode involving two different oligosaccharide-binding sites adopted by STBD1 CBM20 for recognizing glycogen. In addition, we demonstrate that the LC3-interacting region (LIR) motif of STBD1 can selectively bind to six mammalian ATG8 family members. We elucidate the detailed molecular mechanism underlying the selective interactions of STBD1 with ATG8 family proteins by solving the STBD1 LIR/GABARAPL1 complex structure. Importantly, our cell-based assays reveal that both the STBD1 LIR/GABARAPL1 interaction and the intact two oligosaccharide binding sites of STBD1 CBM20 are essential for the effective association of STBD1, GABARAPL1, and glycogen in cells. Finally, through mass spectrometry, biochemical, and structural modeling analyses, we unveil that STBD1 can directly bind to the Claw domain of RB1CC1 through its LIR, thereby recruiting the key autophagy initiation factor RB1CC1. In all, our findings provide mechanistic insights into the recognitions of glycogen, ATG8 family proteins, and RB1CC1 by STBD1 and shed light on the potential working mechanism of STBD1-mediated glycophagy.

PubMed: 39236246
DOI: 10.1073/pnas.2402817121

実験手法

X-RAY DIFFRACTION (1.53 Å)