8X7X

Crystal structure of SADS-CoV fusion core

8X7X の概要

• エントリーDOI: 10.2210/pdb8x7x/pdb
• 分子名称: HR1, HR2, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: fusion core, postfusion, viral protein
• 由来する生物種: Coronaviridae; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 12044.94

構造登録者

Yan, L. (登録日: 2023-11-26, 公開日: 2024-02-21, 最終更新日: 2024-03-13)

主引用文献

Wang, F.,Yang, G.,Yan, L.
Crystal Structures of Fusion Cores from CCoV-HuPn-2018 and SADS-CoV.
Viruses, 16:-, 2024

PubMed Abstract: Cross-species spillover to humans of coronaviruses (CoVs) from wildlife animal reservoirs poses marked and global threats to human and animal health. Recently, sporadic infection of canine coronavirus-human pneumonia-2018 (CCoV-HuPn-2018) in hospitalized patients with pneumonia genetically related to canine and feline coronavirus were identified. In addition, swine acute diarrhea syndrome coronavirus (SADS-CoV) had the capability of broad tropism to cultured cells including from humans. Together, the transmission of Alphacoronaviruses that originated in wildlife to humans via intermediate hosts was responsible for the high-impact emerging zoonosis. Entry of CoV is mainly mediated by Spike and formation of a typical six helix bundle (6-HB) structure in the postfusion state of Spike is pivotal. Here, we present the complete fusion core structures of CCoV-HuPn-2018 and SADS-CoV from Alphacoronavirus at 2.10 and 2.59 Å, respectively. The overall structure of the CCoV-HuPn-2018 fusion core is similar to Alphacoronavirus like HCoV-229E, while SADS-CoV is analogous to Betacoronavirus like SARS-CoV-2. Collectively, we provide a structural basis for the development of pan-CoV small molecules and polypeptides based on the HR1-HR2 complex, concerning CCoV-HuPn-2018 and SADS-CoV.

PubMed: 38400047
DOI: 10.3390/v16020272

実験手法

X-RAY DIFFRACTION (2.59 Å)