8X2D

Crystal structure of H5 hemagglutinin from swan-infecting H5N8 influenza virus complexed with avian receptor analog LSTc

8X2D の概要

• エントリーDOI: 10.2210/pdb8x2d/pdb
• 関連するBIRD辞書のPRD_ID: PRD_900046
• 分子名称: Hemagglutinin, N-acetyl-alpha-neuraminic acid-(2-6)-beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-3)-beta-D-galactopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Influenza A virus
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 177473.97

構造登録者

Jin, X.Y.,Han, P.,Song, H.,Qi, J.X. (登録日: 2023-11-09, 公開日: 2025-05-14, 最終更新日: 2026-07-01)

主引用文献

Jin, X.,Han, P.,Wang, Y.,Wang, H.,Zhang, C.,Di Maio, A.,Yu, J.,Hao, T.,Gu, Y.,Zhang, Z.,Zhang, W.,Qi, J.,Bi, Y.,Zhang, X.,Sun, L.,Wang, N.,Liu, Y.,Song, H.,Gao, G.F.
Hemagglutinin double-mutation enhances binding of human-infecting avian influenza virus clade 2.3.4.4b H5Ny to human and SLe X receptors.
Embo Rep., 2026

PubMed Abstract: Clade 2.3.4.4b H5Ny highly pathogenic avian influenza viruses (HPAIVs) continue to circulate worldwide, posing zoonotic threats, especially with recent cattle outbreaks. The mechanisms by which these viruses adapt to mammalian hosts while maintaining a broad avian tropism remain poorly understood. Here, we demonstrate that two naturally occurring mutations (K222Q and S227R) in the hemagglutinin (HA) of a human-infecting H5N8 strain, first identified in 2020, enhance binding affinity for both α2-6-linked and Sialyl Lewis (SLe) glycans, which may underlie the broad tissue binding and cross-species potential. Structural analyses reveal that these mutations expand receptor specificity for these glycans, which are abundant in the human respiratory tract and duck trachea, providing a possible molecular basis for cross-species transmission. Our findings suggest that clade 2.3.4.4b H5Ny viruses evolved dual receptor specificity as early as the 2020 Russian H5N8 strain, potentially contributing to sporadic human infections and widespread dissemination among birds and mammals.

PubMed: 42303811
DOI: 10.1038/s44319-026-00816-2

実験手法

X-RAY DIFFRACTION (2.48 Å)