8WYS

Local map of human CD5L bound to IgM-Fc/J

8WYS の概要

• エントリーDOI: 10.2210/pdb8wys/pdb
• EMDBエントリー: 37937
• 分子名称: Interleukin-2,Isoform 1 of Immunoglobulin heavy constant mu, Interleukin-2,CD5 antigen-like, Immunoglobulin J chain, ... (6 entities in total)
• 機能のキーワード: immunoglobulin, cd5 antigen-like, pentamer, immune system
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 148082.17

構造登録者

Wang, Y.X.,Su, C.,Xiao, J.Y. (登録日: 2023-10-31, 公開日: 2024-10-09, 最終更新日: 2024-11-13)

主引用文献

Wang, Y.,Su, C.,Ji, C.,Xiao, J.
CD5L associates with IgM via the J chain.
Nat Commun, 15:8397-8397, 2024

PubMed Abstract: CD5 antigen-like (CD5L), also known as Spα or AIM (Apoptosis inhibitor of macrophage), emerges as an integral component of serum immunoglobulin M (IgM). However, the molecular mechanism underlying the interaction between IgM and CD5L has remained elusive. In this study, we present a cryo-electron microscopy structure of the human IgM pentamer core in complex with CD5L. Our findings reveal that CD5L binds to the joining chain (J chain) in a Ca-dependent manner and further links to IgM via a disulfide bond. We further corroborate recently published data that CD5L reduces IgM binding to the mucosal transport receptor pIgR, but does not impact the binding of the IgM-specific receptor FcμR. Additionally, CD5L does not interfere with IgM-mediated complement activation. These results offer a more comprehensive understanding of IgM and shed light on the function of the J chain in the immune system.

PubMed: 39333069
DOI: 10.1038/s41467-024-52175-y

実験手法

ELECTRON MICROSCOPY (3.41 Å)