8WUA

cryo-EM structure of human TMEM63A

8WUA の概要

• エントリーDOI: 10.2210/pdb8wua/pdb
• EMDBエントリー: 37852
• 分子名称: CSC1-like protein 1 (1 entity in total)
• 機能のキーワード: mechanically activated (ma) ion channel, membrane protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 82331.83

構造登録者

Yang, D. (登録日: 2023-10-20, 公開日: 2024-01-24, 最終更新日: 2025-07-02)

主引用文献

Wu, X.,Shang, T.,Lu, X.,Luo, D.,Yang, D.
A monomeric structure of human TMEM63A protein.
Proteins, 92:750-756, 2024

PubMed Abstract: OSCA/TMEM63 is a newly identified family of mechanically activated (MA) ion channels in plants and animals, respectively, which convert physical forces into electrical signals or trigger intracellular cascades and are essential for eukaryotic physiology. OSCAs and related TMEM16s and transmembrane channel-like (TMC) proteins form homodimers with two pores. However, the molecular architecture of the mammalian TMEM63 proteins remains unclear. Here we elucidate the structure of human TMEM63A in the presence of calcium by single particle cryo-EM, revealing a distinct monomeric architecture containing eleven transmembrane helices. It has structural similarity to the single subunit of the Arabidopsis thaliana OSCA proteins. We locate the ion permeation pathway within the monomeric configuration and observe a nonprotein density resembling lipid. These results lay a foundation for understanding the structural organization of OSCA/TMEM63A family proteins.

PubMed: 38217391
DOI: 10.1002/prot.26660

実験手法

ELECTRON MICROSCOPY (3.6 Å)