8WTJ

XBB.1.5.70 spike protein in complex with ACE2

これはPDB形式変換不可エントリーです。

8WTJ の概要

• エントリーDOI: 10.2210/pdb8wtj/pdb
• EMDBエントリー: 37835
• 分子名称: Spike glycoprotein, Processed angiotensin-converting enzyme 2 (2 entities in total)
• 機能のキーワード: sars-cov-2, xbb.1.5.70, ace2, spike protein, viral protein
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 506241.64

構造登録者

Feng, L.L.,Feng, L.L. (登録日: 2023-10-18, 公開日: 2023-12-13, 最終更新日: 2025-07-02)

主引用文献

Jian, F.,Feng, L.,Yang, S.,Yu, Y.,Wang, L.,Song, W.,Yisimayi, A.,Chen, X.,Xu, Y.,Wang, P.,Yu, L.,Wang, J.,Liu, L.,Niu, X.,Wang, J.,Xiao, T.,An, R.,Wang, Y.,Gu, Q.,Shao, F.,Jin, R.,Shen, Z.,Wang, Y.,Wang, X.,Cao, Y.
Convergent evolution of SARS-CoV-2 XBB lineages on receptor-binding domain 455-456 synergistically enhances antibody evasion and ACE2 binding.
Plos Pathog., 19:e1011868-e1011868, 2023

PubMed Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) XBB lineages have achieved dominance worldwide and keep on evolving. Convergent evolution of XBB lineages on the receptor-binding domain (RBD) L455F and F456L is observed, resulting in variants with substantial growth advantages, such as EG.5, FL.1.5.1, XBB.1.5.70, and HK.3. Here, we show that neutralizing antibody (NAb) evasion drives the convergent evolution of F456L, while the epistatic shift caused by F456L enables the subsequent convergence of L455F through ACE2 binding enhancement and further immune evasion. L455F and F456L evade RBD-targeting Class 1 public NAbs, reducing the neutralization efficacy of XBB breakthrough infection (BTI) and reinfection convalescent plasma. Importantly, L455F single substitution significantly dampens receptor binding; however, the combination of L455F and F456L forms an adjacent residue flipping, which leads to enhanced NAbs resistance and ACE2 binding affinity. The perturbed receptor-binding mode leads to the exceptional ACE2 binding and NAb evasion, as revealed by structural analyses. Our results indicate the evolution flexibility contributed by epistasis cannot be underestimated, and the evolution potential of SARS-CoV-2 RBD remains high.

PubMed: 38117863
DOI: 10.1371/journal.ppat.1011868

実験手法

ELECTRON MICROSCOPY (4.64 Å)