8WT8

Cryo-EM structure of the IS621 recombinase in complex with bridge RNA, donor DNA, and target DNA in the post-strand exchange state (Holliday junction intermediate)

Summary for 8WT8

Entry DOI	10.2210/pdb8wt8/pdb
EMDB information	37829
Descriptor	IS621 transposase, bridge RNA, target DNA-donor DNA, ... (7 entities in total)
Functional Keywords	holliday junction, rna dependent recombinase, recombination-rna-dna complex, recombination/rna/dna
Biological source	Escherichia coli More
Total number of polymer chains	10
Total formula weight	312761.97
Authors	Hiraizumi, M.,Yamashita, K.,Nishimasu, H. (deposition date: 2023-10-18, release date: 2024-06-26, Last modification date: 2024-07-17)
Primary citation	Hiraizumi, M.,Perry, N.T.,Durrant, M.G.,Soma, T.,Nagahata, N.,Okazaki, S.,Athukoralage, J.S.,Isayama, Y.,Pai, J.J.,Pawluk, A.,Konermann, S.,Yamashita, K.,Hsu, P.D.,Nishimasu, H. Structural mechanism of bridge RNA-guided recombination. Nature, 630:994-1002, 2024 Cited by PubMed: 38926616 DOI: 10.1038/s41586-024-07570-2 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (2.9 Å)

Structure validation