8WRF

Crystal structure of MexL

8WRF の概要

• エントリーDOI: 10.2210/pdb8wrf/pdb
• 分子名称: Probable transcriptional regulator (1 entity in total)
• 機能のキーワード: tetr family, transcription
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 44382.90

構造登録者

Wei, Y.,Wu, Z.K. (登録日: 2023-10-14, 公開日: 2025-03-05, 最終更新日: 2025-03-12)

主引用文献

Yu, Z.,Wu, Z.,Liu, D.,Liu, H.,Zhang, Y.,Zheng, Y.,Huang, Y.,Liao, S.,Wei, Y.,Huang, W.,Zhang, Z.,Liu, X.,Yu, H.,Wang, D.,Li, L.,Long, F.,Ma, L.Z.
Dual-function regulator MexL as a target to control phenazines production and pathogenesis of Pseudomonas aeruginosa.
Nat Commun, 16:2000-2000, 2025

PubMed Abstract: Antibiotic resistance or tolerance of pathogens has become one of the global public crises. Finding new drug targets may open up a way of infection control. Phenazine pyocyanin (PYO) is an important virulence factor produced by the pathogen Pseudomonas aeruginosa. Here we show that a multidrug efflux pump repressor, MexL, acts as a transcriptional activator to enhance phenazines production via binding with a conserved DNA motif within the promoters of phenazines biosynthesis genes. Moreover, PYO functions as a self-regulating ligand of MexL for restricting its own production and the mexL knockout attenuates the virulence and antibiotics tolerance of P. aeruginosa. Based on the structure of MexL we resolve, we find two antimicrobials that can interact with MexL to reduce the PYO production and virulence of P. aeruginosa. Our in vivo studies suggest that the antimicrobials combination by using MexL-antagonists to reduce bacterial virulence and enhance the efficacy of common antibiotics can be an effective way to combat P. aeruginosa infection.

PubMed: 40011517
DOI: 10.1038/s41467-025-57294-8

実験手法

X-RAY DIFFRACTION (3 Å)