8WQP

Cryo-EM structure of T. pseudonana PyShell helical tube

8WQP の概要

• エントリーDOI: 10.2210/pdb8wqp/pdb
• EMDBエントリー: 37751
• 分子名称: Diatom the pyrenoid shell protein (1 entity in total)
• 機能のキーワード: marine diatoms, photosynthesis, co2-concentrating mechanism, pyrenoids, plant protein
• 由来する生物種: Thalassiosira pseudonana (Marine diatom, Cyclotella nana)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 49972.04

構造登録者

Kawamoto, A.,Tohda, R.,Gerle, C.,Kurisu, G. (登録日: 2023-10-12, 公開日: 2024-10-09, 最終更新日: 2024-11-06)

主引用文献

Shimakawa, G.,Demulder, M.,Flori, S.,Kawamoto, A.,Tsuji, Y.,Nawaly, H.,Tanaka, A.,Tohda, R.,Ota, T.,Matsui, H.,Morishima, N.,Okubo, R.,Wietrzynski, W.,Lamm, L.,Righetto, R.D.,Uwizeye, C.,Gallet, B.,Jouneau, P.H.,Gerle, C.,Kurisu, G.,Finazzi, G.,Engel, B.D.,Matsuda, Y.
Diatom pyrenoids are encased in a protein shell that enables efficient CO 2 fixation.
Cell, 187:5919-, 2024

PubMed Abstract: Pyrenoids are subcompartments of algal chloroplasts that increase the efficiency of Rubisco-driven CO fixation. Diatoms fix up to 20% of global CO, but their pyrenoids remain poorly characterized. Here, we used in vivo photo-crosslinking to identify pyrenoid shell (PyShell) proteins, which we localized to the pyrenoid periphery of model pennate and centric diatoms, Phaeodactylum tricornutum and Thalassiosira pseudonana. In situ cryo-electron tomography revealed that pyrenoids of both diatom species are encased in a lattice-like protein sheath. Single-particle cryo-EM yielded a 2.4-Å-resolution structure of an in vitro TpPyShell1 lattice, which showed how protein subunits interlock. T. pseudonana TpPyShell1/2 knockout mutants had no PyShell sheath, altered pyrenoid morphology, and a high-CO requiring phenotype, with reduced photosynthetic efficiency and impaired growth under standard atmospheric conditions. The structure and function of the diatom PyShell provide a molecular view of how CO is assimilated in the ocean, a critical ecosystem undergoing rapid change.

PubMed: 39357521
DOI: 10.1016/j.cell.2024.09.013

実験手法

ELECTRON MICROSCOPY (2.4 Å)