8WQL

In situ PBS-PSII supercomplex from cyanobacterial Spirulina platensis

これはPDB形式変換不可エントリーです。

8WQL の概要

• エントリーDOI: 10.2210/pdb8wql/pdb
• EMDBエントリー: 37749
• 分子名称: LRC1, Cytochrome b559 subunit alpha, Cytochrome b559 subunit beta, ... (48 entities in total)
• 機能のキーワード: complex, photosynthesis
• 由来する生物種: Arthrospira sp. FACHB-439; 詳細
• タンパク質・核酸の鎖数: 445
• 化学式量合計: 8522496.38

構造登録者

You, X.,Zhang, X.,Xiao, Y.N.,Sun, S.,Sui, S.F. (登録日: 2023-10-11, 公開日: 2024-07-31, 最終更新日: 2025-09-17)

主引用文献

Zhang, X.,Xiao, Y.,You, X.,Sun, S.,Sui, S.F.
In situ structural determination of cyanobacterial phycobilisome-PSII supercomplex by STAgSPA strategy.
Nat Commun, 15:7201-7201, 2024

PubMed Abstract: Photosynthesis converting solar energy to chemical energy is one of the most important chemical reactions on earth. In cyanobacteria, light energy is captured by antenna system phycobilisomes (PBSs) and transferred to photosynthetic reaction centers of photosystem II (PSII) and photosystem I (PSI). While most of the protein complexes involved in photosynthesis have been characterized by in vitro structural analyses, how these protein complexes function together in vivo is not well understood. Here we implemented STAgSPA, an in situ structural analysis strategy, to solve the native structure of PBS-PSII supercomplex from the cyanobacteria Arthrospira sp. FACHB439 at resolution of ~3.5 Å. The structure reveals coupling details among adjacent PBSs and PSII dimers, and the collaborative energy transfer mechanism mediated by multiple super-PBS in cyanobacteria. Our results provide insights into the diversity of photosynthesis-related systems between prokaryotic cyanobacteria and eukaryotic red algae but are also a methodological demonstration for high-resolution structural analysis in cellular or tissue samples.

PubMed: 39169020
DOI: 10.1038/s41467-024-51460-0

実験手法

ELECTRON MICROSCOPY (3.5 Å)