8WPZ

Cryo-ET structure of RuBisCO at 3.9 angstroms from Synechococcus elongatus PCC 7942

8WPZ の概要

• エントリーDOI: 10.2210/pdb8wpz/pdb
• EMDBエントリー: 37727
• 分子名称: Ribulose bisphosphate carboxylase small subunit, Ribulose bisphosphate carboxylase large chain (2 entities in total)
• 機能のキーワード: carboxysome, rubisco, cryo-et, photosynthesis
• 由来する生物種: Synechococcus elongatus PCC 7942 = FACHB-805; 詳細
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 526926.41

構造登録者

Kong, W.W.,Jiang, Y.L.,Zhou, C.Z. (登録日: 2023-10-10, 公開日: 2024-05-15, 最終更新日: 2025-07-23)

主引用文献

Kong, W.W.,Zhu, Y.,Zhao, H.R.,Du, K.,Zhou, R.Q.,Li, B.,Yang, F.,Hou, P.,Huang, X.H.,Chen, Y.,Wang, Y.C.,Sun, F.,Jiang, Y.L.,Zhou, C.Z.
Cryo-electron tomography reveals the packaging pattern of RuBisCOs in Synechococcus beta-carboxysome.
Structure, 32:1110-1120.e4, 2024

PubMed Abstract: Carboxysomes are large self-assembled microcompartments that serve as the central machinery of a CO-concentrating mechanism (CCM). Biogenesis of carboxysome requires the fine organization of thousands of individual proteins; however, the packaging pattern of internal RuBisCOs remains largely unknown. Here we purified the intact β-carboxysomes from Synechococcus elongatus PCC 7942 and identified the protein components by mass spectrometry. Cryo-electron tomography combined with subtomogram averaging revealed the general organization pattern of internal RuBisCOs, in which the adjacent RuBisCOs are mainly arranged in three distinct manners: head-to-head, head-to-side, and side-by-side. The RuBisCOs in the outermost layer are regularly aligned along the shell, the majority of which directly interact with the shell. Moreover, statistical analysis enabled us to propose an ideal packaging model of RuBisCOs in the β-carboxysome. These results provide new insights into the biogenesis of β-carboxysomes and also advance our understanding of the efficient carbon fixation functionality of carboxysomes.

PubMed: 38823379
DOI: 10.1016/j.str.2024.05.007

実験手法

ELECTRON MICROSCOPY (3.9 Å)