8WCL
FCP pentamer in Chaetoceros gracilis
Summary for 8WCL
| Entry DOI | 10.2210/pdb8wcl/pdb |
| EMDB information | 37442 |
| Descriptor | Chlorophyll a/c-binding protein Lhcf6, 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE, Chlorophyll a/b-binding protein, ... (10 entities in total) |
| Functional Keywords | fcp pentamer in chaetoceros gracilis, photosynthesis |
| Biological source | Chaetoceros neogracilis More |
| Total number of polymer chains | 5 |
| Total formula weight | 171181.23 |
| Authors | |
| Primary citation | Zhou, C.,Feng, Y.,Li, Z.,Shen, L.,Li, X.,Wang, Y.,Han, G.,Kuang, T.,Liu, C.,Shen, J.R.,Wang, W. Structural and spectroscopic insights into fucoxanthin chlorophyll a/c-binding proteins of diatoms in diverse oligomeric states. Plant Commun., 5:101041-101041, 2024 Cited by PubMed Abstract: Diatoms, a group of prevalent marine algae, significantly contribute to global primary productivity. Their substantial biomass is linked to enhanced absorption of blue-green light underwater, facilitated by fucoxanthin chlorophyll a/c-binding proteins (FCPs), exhibiting oligomeric diversity across diatom species. Utilizing mild CN-PAGE analysis on solubilized thylakoid membranes, we displayed monomeric, dimeric, trimeric, tetrameric and pentameric FCPs in diatoms. Mass spectrometry analysis revealed each oligomeric FCP has specific protein compositions, constituting a large Lhcf family of FCP antennas. In addition, we resolved the structures of Thalassiosira pseudonana FCP (Tp-FCP) homotrimer and Chaetoceros gracilis FCP (Cg-FCP) pentamer by cryo-electron microscopy at 2.73 Å and 2.65 Å resolutions, respectively. The distinct pigment composition and organization in various oligomeric FCPs change their blue-green light-harvesting, excitation energy transfer pathways. In comparison to dimeric and trimeric FCPs, Cg-FCP tetramer and Cg-FCP pentamer exhibit stronger absorption by Chls c, red-shifted and broader Chl a fluorescence emission, as well as more robust circular dichroism signals originating from Chl a-carotenoid dimers. These spectroscopic characteristics indicate that Chl a molecules in Cg-FCP tetramer and Cg-FCP pentamer are more heterogeneous than in both dimers and Tp-FCP trimer. The structural and spectroscopic insights provided by this study contribute to a better understanding of the mechanisms that empower diatoms to adapt to fluctuating light environments. PubMed: 39030906DOI: 10.1016/j.xplc.2024.101041 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.65 Å) |
Structure validation
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