8WB7

CryoEM structure of Snf7 N-terminal domain in the inner coils of spiral

8WB7 の概要

• エントリーDOI: 10.2210/pdb8wb7/pdb
• EMDBエントリー: 37417
• 分子名称: SNF7 isoform 1 (1 entity in total)
• 機能のキーワード: membrane fission, spiral polymers, n-terminal domain, "open" status, protein transport
• 由来する生物種: Saccharomyces cerevisiae (brewer's yeast)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27020.12

構造登録者

Liu, M.D.,Shen, Q.T. (登録日: 2023-09-08, 公開日: 2024-05-29, 最終更新日: 2024-06-05)

主引用文献

Liu, M.,Liu, Y.,Song, T.,Yang, L.,Qi, L.,Zhang, Y.Z.,Wang, Y.,Shen, Q.T.
Three-dimensional architecture of ESCRT-III flat spirals on the membrane.
Proc.Natl.Acad.Sci.USA, 121:e2319115121-e2319115121, 2024

PubMed Abstract: The endosomal sorting complexes required for transport (ESCRTs) are responsible for membrane remodeling in many cellular processes, such as multivesicular body biogenesis, viral budding, and cytokinetic abscission. ESCRT-III, the most abundant ESCRT subunit, assembles into flat spirals as the primed state, essential to initiate membrane invagination. However, the three-dimensional architecture of ESCRT-III flat spirals remained vague for decades due to highly curved filaments with a small diameter and a single preferred orientation on the membrane. Here, we unveiled that yeast Snf7, a component of ESCRT-III, forms flat spirals on the lipid monolayers using cryogenic electron microscopy. We developed a geometry-constrained Euler angle-assigned reconstruction strategy and obtained moderate-resolution structures of Snf7 flat spirals with varying curvatures. Our analyses showed that Snf7 subunits recline on the membrane with N-terminal motifs α0 as anchors, adopt an open state with fused α2/3 helices, and bend α2/3 gradually from the outer to inner parts of flat spirals. In all, we provide the orientation and conformations of ESCRT-III flat spirals on the membrane and unveil the underlying assembly mechanism, which will serve as the initial step in understanding how ESCRTs drive membrane abscission.

PubMed: 38709931
DOI: 10.1073/pnas.2319115121

実験手法

ELECTRON MICROSCOPY (7.4 Å)