8WB3
Human H Chain Ferritin mutant-K86Q with 2 Fe(III)/subunit loading
Summary for 8WB3
| Entry DOI | 10.2210/pdb8wb3/pdb |
| Descriptor | Ferritin heavy chain, FE (III) ION, CALCIUM ION, ... (4 entities in total) |
| Functional Keywords | enzyme, metal binding protein |
| Biological source | Homo sapiens (human) |
| Total number of polymer chains | 1 |
| Total formula weight | 21486.53 |
| Authors | |
| Primary citation | Jiao, R.,Zhao, G.,Zhang, T. Structural Insights into the Reaction between Hydrogen Peroxide and Di-iron Complexes at the Ferroxidase Center of Ferritin. Inorg.Chem., 63:3359-3365, 2024 Cited by PubMed Abstract: The Fe(II) oxidation mechanism in the ferroxidase center of heavy chain ferritin has been studied extensively. However, the actual production of HO was found to be substantially lower than expected at low flux of Fe(II) to ferritin subunits. Here, we demonstrated that HO could interact with the di-iron nuclear center, leading to the production of hydroxyl radicals and oxygen. Two reaction intermediates were captured in the ferroxidase center by using the time-lapse crystallographic techniques in a shellfish ferritin. The crystal structures revealed the binding of HO as a μ -1,2-peroxo-diferric species and the binding of O to the diferric structure. This investigation sheds light on the reaction between the di-iron nuclear center and HO and provides insights for the exploitation of metalloenzymes. PubMed: 38315811DOI: 10.1021/acs.inorgchem.3c03889 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.49 Å) |
Structure validation
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