8WAY

De novo transcribing complex 15 (TC15), the early elongation complex with Pol II positioned 15nt downstream of TSS

Summary for 8WAY

• Entry DOI: 10.2210/pdb8way/pdb
• Related: 7EGB 8WAK 8WAL 8WAN 8WAO 8WAP 8WAQ 8WAR 8WAS 8WAT 8WAU 8WAV 8WAW 8WAX 8WAZ 8WB0
• EMDB information: 31111 37395 37396 37398 37399 37400 37401 37402 37403 37404 37405 37406 37407 37408 37409 37410 37411 37412 37413
• Related PRD ID: PRD_000201
• Descriptor: Alpha-amanitin, DNA-directed RNA polymerase II subunit RPB4, DNA-directed RNA polymerase II subunit E, ... (21 entities in total)
• Functional Keywords: transcribing complex, de novo transcription initiation, early elongation complex (eec), transcription
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 18
• Total formula weight: 671383.81

Authors

Chen, X.,Liu, W.,Wang, Q.,Wang, X.,Ren, Y.,Qu, X.,Li, W.,Xu, Y. (deposition date: 2023-09-08, release date: 2023-12-06, Last modification date: 2024-01-03)

Primary citation

Chen, X.,Liu, W.,Wang, Q.,Wang, X.,Ren, Y.,Qu, X.,Li, W.,Xu, Y.
Structural visualization of transcription initiation in action.
Science, 382:eadi5120-eadi5120, 2023

PubMed Abstract: Transcription initiation is a complex process, and its mechanism is incompletely understood. We determined the structures of de novo transcribing complexes TC2 to TC17 with RNA polymerase II halted on G-less promoters when nascent RNAs reach 2 to 17 nucleotides in length, respectively. Connecting these structures generated a movie and a working model. As initially synthesized RNA grows, general transcription factors (GTFs) remain bound to the promoter and the transcription bubble expands. Nucleoside triphosphate (NTP)-driven RNA-DNA translocation and template-strand accumulation in a nearly sealed channel may promote the transition from initially transcribing complexes (ITCs) (TC2 to TC9) to early elongation complexes (EECs) (TC10 to TC17). Our study shows dynamic processes of transcription initiation and reveals why ITCs require GTFs and bubble expansion for initial RNA synthesis, whereas EECs need GTF dissociation from the promoter and bubble collapse for promoter escape.

PubMed: 38127763
DOI: 10.1126/science.adi5120

Experimental method

ELECTRON MICROSCOPY (2.85 Å)