8WA0

The cryo-EM structure of the Nicotiana tabacum PEP-PAP-TEC1

これはPDB形式変換不可エントリーです。

8WA0 の概要

• エントリーDOI: 10.2210/pdb8wa0/pdb
• EMDBエントリー: 37387
• 分子名称: DNA-directed RNA polymerase subunit alpha, Fructokinase-like 1, chloroplastic, Fructokinase-like 2, chloroplastic, ... (23 entities in total)
• 機能のキーワード: transcription, pep, pap
• 由来する生物種: Nicotiana tabacum (common tobacco); 詳細
• タンパク質・核酸の鎖数: 22
• 化学式量合計: 1245490.48

構造登録者

Wu, X.X.,Zhang, Y. (登録日: 2023-09-06, 公開日: 2024-03-13, 最終更新日: 2024-11-13)

主引用文献

Wu, X.X.,Mu, W.H.,Li, F.,Sun, S.Y.,Cui, C.J.,Kim, C.,Zhou, F.,Zhang, Y.
Cryo-EM structures of the plant plastid-encoded RNA polymerase.
Cell, 187:1127-1144.e21, 2024

PubMed Abstract: Chloroplasts are green plastids in the cytoplasm of eukaryotic algae and plants responsible for photosynthesis. The plastid-encoded RNA polymerase (PEP) plays an essential role during chloroplast biogenesis from proplastids and functions as the predominant RNA polymerase in mature chloroplasts. The PEP-centered transcription apparatus comprises a bacterial-origin PEP core and more than a dozen eukaryotic-origin PEP-associated proteins (PAPs) encoded in the nucleus. Here, we determined the cryo-EM structures of Nicotiana tabacum (tobacco) PEP-PAP apoenzyme and PEP-PAP transcription elongation complexes at near-atomic resolutions. Our data show the PEP core adopts a typical fold as bacterial RNAP. Fifteen PAPs bind at the periphery of the PEP core, facilitate assembling the PEP-PAP supercomplex, protect the complex from oxidation damage, and likely couple gene transcription with RNA processing. Our results report the high-resolution architecture of the chloroplast transcription apparatus and provide the structural basis for the mechanistic and functional study of transcription regulation in chloroplasts.

PubMed: 38428393
DOI: 10.1016/j.cell.2024.01.026

実験手法

ELECTRON MICROSCOPY (2.7 Å)