8W93

Azumapecten Farreri homopolymeric ferritin (ApF) mutant-H2KE

8W93 の概要

• エントリーDOI: 10.2210/pdb8w93/pdb
• 分子名称: Ferritin, FE (III) ION (3 entities in total)
• 機能のキーワード: enzyme, metal binding protein
• 由来する生物種: Azumapecten farreri
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20072.02

構造登録者

Zhang, T.,Jiao, R. (登録日: 2023-09-04, 公開日: 2024-09-11, 最終更新日: 2025-03-26)

主引用文献

Jiao, R.,Zhao, G.,Zhang, T.
Structural Insights into the Reaction between Hydrogen Peroxide and Di-iron Complexes at the Ferroxidase Center of Ferritin.
Inorg.Chem., 63:3359-3365, 2024

PubMed Abstract: The Fe(II) oxidation mechanism in the ferroxidase center of heavy chain ferritin has been studied extensively. However, the actual production of HO was found to be substantially lower than expected at low flux of Fe(II) to ferritin subunits. Here, we demonstrated that HO could interact with the di-iron nuclear center, leading to the production of hydroxyl radicals and oxygen. Two reaction intermediates were captured in the ferroxidase center by using the time-lapse crystallographic techniques in a shellfish ferritin. The crystal structures revealed the binding of HO as a μ -1,2-peroxo-diferric species and the binding of O to the diferric structure. This investigation sheds light on the reaction between the di-iron nuclear center and HO and provides insights for the exploitation of metalloenzymes.

PubMed: 38315811
DOI: 10.1021/acs.inorgchem.3c03889

実験手法

X-RAY DIFFRACTION (2.09 Å)