8W2V

Solution Structure of the CD28 hinge used in chimeric antigen receptor (CAR) T-cells

Summary for 8W2V

• Entry DOI: 10.2210/pdb8w2v/pdb
• NMR Information: BMRB: 31150
• Descriptor: T-cell-specific surface glycoprotein CD28 (1 entity in total)
• Functional Keywords: car-t, cd28, immunotherapy, intrinsic disorder, immune system
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 1
• Total formula weight: 4308.03

Authors

Folimonova, V.,Chen, X.,Walters, K.J. (deposition date: 2024-02-21, release date: 2024-09-11)

Primary citation

Folimonova, V.,Chen, X.,Negi, H.,Schwieters, C.D.,Li, J.,Byrd, R.A.,Taylor, N.,Youkharibache, P.,Walters, K.J.
CD28 hinge used in chimeric antigen receptor (CAR) T-cells exhibits local structure and conformational exchange amidst global disorder.
Commun Biol, 7:1072-1072, 2024

PubMed Abstract: T-cell therapies based on chimeric antigen receptor (CAR) targeting of a tumor-specific antigen offer hope for patients with relapsed or refractory cancers. CAR hinge and transmembrane regions link antigen recognition domains to intracellular signal transduction domains. Here, we apply biophysical methods to characterize the structure and dynamic properties of the CD28 CAR hinge (CD28H) used in an FDA-approved CD19 CAR for the treatment of B-lineage leukemia/lymphoma. By using nuclear Overhauser effect spectroscopy (NOESY), which detects even transiently occupied structural motifs, we observed otherwise elusive local structural elements amidst overall disorder in CD28H, including a conformational switch from a native β-strand to a 3-helix and polyproline II helix-like structure. These local structural motifs contribute to an overall loosely formed extended geometry that could be captured by NOESY data. All FDA-approved CARs use prolines in the hinge region, which we find in CD28, and previously in CD8α, isomerize to promote structural plasticity and dynamics. These local structural elements may function in recognition and signaling events and constrain the spacing between the transmembrane and antigen recognition domains. Our study thus demonstrates a method for detecting local and transient structure within intrinsically disordered systems and moreover, our CD28H findings may inform future CAR design.

PubMed: 39217198
DOI: 10.1038/s42003-024-06770-w

Experimental method

SOLUTION NMR