8W22

Umb1 umbrella toxin particle (local refinement of UmbB1 bound ALF of UmbC1 and UmbA1)

8W22 の概要

• エントリーDOI: 10.2210/pdb8w22/pdb
• EMDBエントリー: 43737
• 分子名称: Intein C-terminal splicing domain-containing protein, Secreted protein, Secreted esterase (3 entities in total)
• 機能のキーワード: streptomyces, umbrella toxin particles, alanine leucine phenylalanine-rich (alf) repeat proteins, seattle structural genomics center for infectious disease, ssgcid, toxin
• 由来する生物種: Streptomyces coelicolor A3(2); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 214340.11

構造登録者

Park, Y.J.,Zhao, Q.,Seattle Structural Genomics Center for Infectious Disease (SSGCID),DiMaio, F.,Mougous, J.D.,Veesler, D. (登録日: 2024-02-19, 公開日: 2024-04-17, 最終更新日: 2024-10-23)

主引用文献

Zhao, Q.,Bertolli, S.,Park, Y.J.,Tan, Y.,Cutler, K.J.,Srinivas, P.,Asfahl, K.L.,Fonesca-Garcia, C.,Gallagher, L.A.,Li, Y.,Wang, Y.,Coleman-Derr, D.,DiMaio, F.,Zhang, D.,Peterson, S.B.,Veesler, D.,Mougous, J.D.
Streptomyces umbrella toxin particles block hyphal growth of competing species.
Nature, 629:165-173, 2024

PubMed Abstract: Streptomyces are a genus of ubiquitous soil bacteria from which the majority of clinically utilized antibiotics derive. The production of these antibacterial molecules reflects the relentless competition Streptomyces engage in with other bacteria, including other Streptomyces species. Here we show that in addition to small-molecule antibiotics, Streptomyces produce and secrete antibacterial protein complexes that feature a large, degenerate repeat-containing polymorphic toxin protein. A cryo-electron microscopy structure of these particles reveals an extended stalk topped by a ringed crown comprising the toxin repeats scaffolding five lectin-tipped spokes, which led us to name them umbrella particles. Streptomyces coelicolor encodes three umbrella particles with distinct toxin and lectin composition. Notably, supernatant containing these toxins specifically and potently inhibits the growth of select Streptomyces species from among a diverse collection of bacteria screened. For one target, Streptomyces griseus, inhibition relies on a single toxin and that intoxication manifests as rapid cessation of vegetative hyphal growth. Our data show that Streptomyces umbrella particles mediate competition among vegetative mycelia of related species, a function distinct from small-molecule antibiotics, which are produced at the onset of reproductive growth and act broadly. Sequence analyses suggest that this role of umbrella particles extends beyond Streptomyces, as we identified umbrella loci in nearly 1,000 species across Actinobacteria.

PubMed: 38632398
DOI: 10.1038/s41586-024-07298-z

実験手法

ELECTRON MICROSCOPY (4 Å)