8W1E

Crystal Structure of DPS-like protein PA4880 from Pseudomonas aeruginosa (dodecamer)

8W1E の概要

• エントリーDOI: 10.2210/pdb8w1e/pdb
• 分子名称: DPS-LIKE PROTEIN, SULFATE ION, FE (II) ION (3 entities in total)
• 機能のキーワード: pa4880, dps protein, metal binding, dna cleavage, dodecamer, metal binding protein
• 由来する生物種: Pseudomonas aeruginosa PAO1
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 247409.88

構造登録者

Lovell, S.,Liu, L.,Seibold, S.,Battaile, K.P.,Rivera, M. (登録日: 2024-02-15, 公開日: 2024-05-29, 最終更新日: 2024-06-19)

主引用文献

Rajapaksha, N.,Yao, H.,Cook, A.,Seibold, S.,Liu, L.,Battaile, K.P.,Fontenot, L.,Donnarumma, F.,Lovell, S.,Rivera, M.
Pseudomonas aeruginosa gene PA4880 encodes a Dps-like protein with a Dps fold, bacterioferritin-type ferroxidase centers, and endonuclease activity.
Front Mol Biosci, 11:1390745-1390745, 2024

PubMed Abstract: We report the biochemical, structural, and functional characterization of the protein coded by gene PA4880 in the PAO1 genome. The PA4880 gene had been annotated as coding a probable bacterioferritin. Our structural work shows that the product of gene PA4880 is a protein that adopts the Dps subunit fold, which oligomerizes into a 12-mer quaternary structure. Unlike Dps, however, the ferroxidase di-iron centers and iron coordinating ligands are buried within each subunit, in a manner identical to that observed in the ferroxidase center of bacterioferritin. Since these structural characteristics correspond to Dps-like proteins, we term the protein as Dps-like, or Pa DpsL. The ferroxidase centers in Pa DpsL catalyze the oxidation of Fe utilizing O or HO as oxidant, and the resultant Fe is compartmentalized in the interior cavity. Interestingly, incubating Pa DpsL with plasmid DNA results in efficient nicking of the DNA and at higher concentrations of Pa DpsL the DNA is linearized and eventually degraded. The nickase and endonuclease activities suggest that Pa DpsL, in addition to participating in the defense of cells against iron-induced toxicity, may also participate in the innate immune mechanisms consisting of restriction endonucleases and cognate methyl transferases.

PubMed: 38841187
DOI: 10.3389/fmolb.2024.1390745

実験手法

X-RAY DIFFRACTION (2.9 Å)