8VZY

Q108K:K40L:T51V:T53S:R58Y mutant of hCRBPII bound to synthetic fluorophore TD-1V

これはPDB形式変換不可エントリーです。

8VZY の概要

• エントリーDOI: 10.2210/pdb8vzy/pdb
• 分子名称: Retinol-binding protein 2, (2E)-3-{5-[4-(dimethylamino)phenyl]thiophen-2-yl}but-2-enal, ACETATE ION, ... (4 entities in total)
• 機能のキーワード: human cellular retinol binding protein ii, hcrbpii, engineered protein, fluorescent protein, retinol binding protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 31475.35

構造登録者

Nossoni, Z.,Bingham, C.R.,Geiger, J.H.,Borhan, B. (登録日: 2024-02-13, 公開日: 2024-04-03, 最終更新日: 2024-11-13)

主引用文献

Santos, E.M.,Chandra, I.,Assar, Z.,Sheng, W.,Ghanbarpour, A.,Bingham, C.,Vasileiou, C.,Geiger, J.H.,Borhan, B.
Regulation of Absorption and Emission in a Protein/Fluorophore Complex.
Acs Chem.Biol., 19:1725-1732, 2024

PubMed Abstract: Human cellular retinol binding protein II (hCRBPII) was used as a protein engineering platform to rationally regulate absorptive and emissive properties of a covalently bound fluorogenic dye. We demonstrate the binding of a thio-dapoxyl analog via formation of a protonated imine between an active site lysine residue and the chromophore's aldehyde. Rational manipulation of the electrostatics of the binding pocket results in a 204 nm shift in absorption and a 131 nm shift in emission. The protein is readily expressed in mammalian systems and binds with exogenously delivered fluorophore as demonstrated by live-cell imaging experiments.

PubMed: 39046136
DOI: 10.1021/acschembio.4c00125

実験手法

X-RAY DIFFRACTION (1.34 Å)