8VYL

The structure of Human Hemoglobin in Complex with Nanobody BtNbE11

8VYL の概要

• エントリーDOI: 10.2210/pdb8vyl/pdb
• 分子名称: Hemoglobin subunit alpha, Hemoglobin subunit beta, Nanobody BtNbE11, ... (6 entities in total)
• 機能のキーワード: hemoglobin, nanobody, oxygen transport
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 95204.96

構造登録者

Grinter, R.,Binks, S.,Fox, D. (登録日: 2024-02-08, 公開日: 2024-07-17, 最終更新日: 2024-10-09)

主引用文献

Fox, D.R.,Samuels, I.,Binks, S.,Grinter, R.
The structure of a haemoglobin-nanobody complex reveals human beta-subunit-specific interactions.
Febs Lett., 598:2240-2248, 2024

PubMed Abstract: Haemoglobin (Hb) is a vital oxygen carrier in vertebrates. Low blood Hb levels may indicate anaemia or genetic disorders, while its presence in the lower digestive system suggests colon cancer. Detecting and quantifying human Hb is essential for medical diagnostics. A nanobody-based sandwich-ELISA test was recently developed utilising llama-derived nanobodies NbE11 and NbB9. These nanobodies specifically bind to human Hb without cross-reacting with Hb from other vertebrates. Here, we determine the crystal structure of NbE11 in complex with human Hb. NbE11 binds Hb with high affinity, predominantly binding the β-Hb subunit. Structural differences between human Hb and other vertebrates at the NbE11 binding interface likely explain the assay's lack of cross-reactivity, providing insights for developing Hb binding diagnostics.

PubMed: 38880764
DOI: 10.1002/1873-3468.14958

実験手法

X-RAY DIFFRACTION (2.02 Å)