8VUH

Human GluN1-2A IgG 003-102 splayed conformation

8VUH の概要

• エントリーDOI: 10.2210/pdb8vuh/pdb
• EMDBエントリー: 43530
• 分子名称: Glutamate receptor ionotropic, NMDA 1, Glutamate receptor ionotropic, NMDA 2A, 003-102 Heavy, ... (5 entities in total)
• 機能のキーワード: receptor, antibody, ion channel, membrane-immune system complex, membrane/immune system
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 413606.77

構造登録者

Michalski, K.,Furukawa, H. (登録日: 2024-01-29, 公開日: 2024-09-11, 最終更新日: 2024-09-18)

主引用文献

Michalski, K.,Abdulla, T.,Kleeman, S.,Schmidl, L.,Gomez, R.,Simorowski, N.,Vallese, F.,Pruss, H.,Heckmann, M.,Geis, C.,Furukawa, H.
Structural and functional mechanisms of anti-NMDAR autoimmune encephalitis.
Nat.Struct.Mol.Biol., 2024

PubMed Abstract: Autoantibodies against neuronal membrane proteins can manifest in autoimmune encephalitis, inducing seizures, cognitive dysfunction and psychosis. Anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis is the most dominant autoimmune encephalitis; however, insights into how autoantibodies recognize and alter receptor functions remain limited. Here we determined structures of human and rat NMDARs bound to three distinct patient-derived antibodies using single-particle electron cryo-microscopy. These antibodies bind different regions within the amino-terminal domain of the GluN1 subunit. Through electrophysiology, we show that all three autoantibodies acutely and directly reduced NMDAR channel functions in primary neurons. Antibodies show different stoichiometry of binding and antibody-receptor complex formation, which in one antibody, 003-102, also results in reduced synaptic localization of NMDARs. These studies demonstrate mechanisms of diverse epitope recognition and direct channel regulation of anti-NMDAR autoantibodies underlying autoimmune encephalitis.

PubMed: 39227719
DOI: 10.1038/s41594-024-01386-4

実験手法

ELECTRON MICROSCOPY (4.42 Å)