8VQR

Crystal structure of chimeric SARS-CoV-2 RBD complexed with chimeric raccoon dog ACE2

8VQR の概要

• エントリーDOI: 10.2210/pdb8vqr/pdb
• 分子名称: Angiotensin-converting enzyme,Processed angiotensin-converting enzyme 2, CHLORIDE ION, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (13 entities in total)
• 機能のキーワード: sars2, cell invasion, hydrolase-viral protein complex, hydrolase, hydrolase/viral protein
• 由来する生物種: Nyctereutes procyonoides (raccoon dog); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 197475.04

構造登録者

Hsueh, F.-C.,Shi, K.,Aihara, H.,Li, F. (登録日: 2024-01-19, 公開日: 2024-05-01, 最終更新日: 2024-11-13)

主引用文献

Hsueh, F.C.,Shi, K.,Mendoza, A.,Bu, F.,Zhang, W.,Aihara, H.,Li, F.
Structural basis for raccoon dog receptor recognition by SARS-CoV-2.
Plos Pathog., 20:e1012204-e1012204, 2024

PubMed Abstract: Since the COVID-19 outbreak, raccoon dogs have been suggested as a potential intermediary in transmitting SARS-CoV-2 to humans. To understand their role in the COVID-19 pandemic and the species barrier for SARS-CoV-2 transmission to humans, we analyzed how their ACE2 protein interacts with SARS-CoV-2 spike protein. Biochemical data showed that raccoon dog ACE2 is an effective receptor for SARS-CoV-2 spike protein, though not as effective as human ACE2. Structural comparisons highlighted differences in the virus-binding residues of raccoon dog ACE2 compared to human ACE2 (L24Q, Y34H, E38D, T82M, R353K), explaining their varied effectiveness as receptors for SARS-CoV-2. These variations contribute to the species barrier that exists between raccoon dogs and humans regarding SARS-CoV-2 transmission. Identifying these barriers can help assess the susceptibility of other mammals to SARS-CoV-2. Our research underscores the potential of raccoon dogs as SARS-CoV-2 carriers and identifies molecular barriers that affect the virus's ability to jump between species.

PubMed: 38709834
DOI: 10.1371/journal.ppat.1012204

実験手法

X-RAY DIFFRACTION (2.565 Å)