8VQK

YcjN from Escherichia coli

8VQK の概要

• エントリーDOI: 10.2210/pdb8vqk/pdb
• 分子名称: Putative ABC transporter periplasmic-binding protein YcjN, SULFATE ION, GLYCEROL, ... (7 entities in total)
• 機能のキーワード: substrate binding protein, abc transporter cognate binding protein, ligand binding protein, unknown function
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 97451.00

構造登録者

Trevino, M.A.,Fernandez, D.,Sharaf, N.G. (登録日: 2024-01-18, 公開日: 2024-10-09, 最終更新日: 2024-11-13)

主引用文献

Trevino, M.A.,Amankwah, K.A.,Fernandez, D.,Weston, S.A.,Stewart, C.J.,Gallardo, J.M.,Shahgholi, M.,Sharaf, N.G.
Expression, purification, and characterization of diacylated Lipo-YcjN from Escherichia coli.
J.Biol.Chem., 300:107853-107853, 2024

PubMed Abstract: YcjN is a putative substrate binding protein expressed from a cluster of genes involved in carbohydrate import and metabolism in Escherichia coli. Here, we determine the crystal structure of YcjN to a resolution of 1.95 Å, revealing that its three-dimensional structure is similar to substrate binding proteins in subcluster D-I, which includes the well-characterized maltose binding protein. Furthermore, we found that recombinant overexpression of YcjN results in the formation of a lipidated form of YcjN that is posttranslationally diacylated at cysteine 21. Comparisons of size-exclusion chromatography profiles and dynamic light scattering measurements of lipidated and nonlipidated YcjN proteins suggest that lipidated YcjN aggregates in solution via its lipid moiety. Additionally, bioinformatic analysis indicates that YcjN-like proteins may exist in both Bacteria and Archaea, potentially in both lipidated and nonlipidated forms. Together, our results provide a better understanding of the aggregation properties of recombinantly expressed bacterial lipoproteins in solution and establish a foundation for future studies that aim to elucidate the role of these proteins in bacterial physiology.

PubMed: 39362470
DOI: 10.1016/j.jbc.2024.107853

実験手法

X-RAY DIFFRACTION (1.95 Å)