8VNQ

Homing endonuclease H98A I-PpoI-DNA complex at pH6.0 (K+ MES) with 1 mM Mn2+ for 1800s

Summary for 8VNQ

• Entry DOI: 10.2210/pdb8vnq/pdb
• Descriptor: DNA (5'-D(*TP*TP*GP*AP*CP*TP*CP*TP*CP*TP*TP*AP*AP*GP*AP*GP*AP*GP*TP*CP*A)-3'), Intron-encoded endonuclease I-PpoI, SODIUM ION, ... (6 entities in total)
• Functional Keywords: intron encoded homing endonuclease i-ppoi, hydrolase
• Biological source: Physarum polycephalum; More
• Total number of polymer chains: 4
• Total formula weight: 48868.41

Authors

Chang, C.,Gao, Y. (deposition date: 2024-01-13, release date: 2024-07-10, Last modification date: 2025-01-29)

Primary citation

Chang, C.,Zhou, G.,Gao, Y.
Observing one-divalent-metal-ion-dependent and histidine-promoted His-Me family I-PpoI nuclease catalysis in crystallo.
Elife, 13:-, 2024

PubMed Abstract: Metal-ion-dependent nucleases play crucial roles in cellular defense and biotechnological applications. Time-resolved crystallography has resolved catalytic details of metal-ion-dependent DNA hydrolysis and synthesis, uncovering the essential roles of multiple metal ions during catalysis. The histidine-metal (His-Me) superfamily nucleases are renowned for binding one divalent metal ion and requiring a conserved histidine to promote catalysis. Many His-Me family nucleases, including homing endonucleases and Cas9 nuclease, have been adapted for biotechnological and biomedical applications. However, it remains unclear how the single metal ion in His-Me nucleases, together with the histidine, promotes water deprotonation, nucleophilic attack, and phosphodiester bond breakage. By observing DNA hydrolysis with His-Me I-PpoI nuclease as a model system, we proved that only one divalent metal ion is required during its catalysis. Moreover, we uncovered several possible deprotonation pathways for the nucleophilic water. Interestingly, binding of the single metal ion and water deprotonation are concerted during catalysis. Our results reveal catalytic details of His-Me nucleases, which is distinct from multi-metal-ion-dependent DNA polymerases and nucleases.

PubMed: 39141555
DOI: 10.7554/eLife.99960

Experimental method

X-RAY DIFFRACTION (1.93 Å)