8VLL

Crystal structure of the yeast cytosine deaminase (yCD) M100W mutant

8VLL の概要

• エントリーDOI: 10.2210/pdb8vll/pdb
• 関連するPDBエントリー: 8VLJ 8VLK 8VLM
• 分子名称: Cytosine deaminase, ZINC ION, PHOSPHATE ION, ... (5 entities in total)
• 機能のキーワード: cytosine deaminase, resistance, heterodimer, antifungal protein
• 由来する生物種: Saccharomyces cerevisiae (brewer's yeast)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 36456.95

構造登録者

Picard, M.-E.,Grenier, J.,Despres, P.C.,Dube, A.K.,Landry, C.R.,Shi, R. (登録日: 2024-01-11, 公開日: 2024-08-21, 最終更新日: 2024-09-18)

主引用文献

Despres, P.C.,Dube, A.K.,Picard, M.E.,Grenier, J.,Shi, R.,Landry, C.R.
Compensatory mutations potentiate constructive neutral evolution by gene duplication.
Science, 385:770-775, 2024

PubMed Abstract: The functions of proteins generally depend on their assembly into complexes. During evolution, some complexes have transitioned from homomers encoded by a single gene to heteromers encoded by duplicate genes. This transition could occur without adaptive evolution through intermolecular compensatory mutations. Here, we experimentally duplicated and evolved a homodimeric enzyme to determine whether and how this could happen. We identified hundreds of deleterious mutations that inactivate individual homodimers but produce functional enzymes when coexpressed as duplicated proteins that heterodimerize. The structure of one such heteromer reveals how both losses of function are buffered through the introduction of asymmetry in the complex that allows them to subfunctionalize. Constructive neutral evolution can thus occur by gene duplication followed by only one deleterious mutation per duplicate.

PubMed: 39146405
DOI: 10.1126/science.ado5719

実験手法

X-RAY DIFFRACTION (1.67 Å)