8VIS

Human TMPRSS11D complexed with a disulfide-linked autoinhibitory DDDDK peptide

Summary for 8VIS

• Entry DOI: 10.2210/pdb8vis/pdb
• Descriptor: Transmembrane protease serine 11D non-catalytic chain, Transmembrane protease serine 11D catalytic chain, MAGNESIUM ION, ... (7 entities in total)
• Functional Keywords: human protease, viral entry, structural genomics, structural genomics consortium, sgc, hydrolase
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 8
• Total formula weight: 173478.44

Authors

Fraser, B.J.,Dong, A.,Ilyassov, O.,Kenney, T.,Li, Y.Y.,Seitova, A.,Li, Y.,Hejazi, Z.,Edwards, A.,Benard, F.,Arrowsmith, C. (deposition date: 2024-01-05, release date: 2024-01-31, Last modification date: 2025-05-28)

Primary citation

Fraser, B.J.,Wilson, R.P.,Ferkova, S.,Ilyassov, O.,Lac, J.,Dong, A.,Li, Y.Y.,Seitova, A.,Li, Y.,Hejazi, Z.,Kenney, T.M.G.,Penn, L.Z.,Edwards, A.,Leduc, R.,Boudreault, P.L.,Morin, G.B.,Benard, F.,Arrowsmith, C.H.
Structural basis of TMPRSS11D specificity and autocleavage activation.
Nat Commun, 16:4351-4351, 2025

PubMed Abstract: Transmembrane Protease, Serine-2 (TMPRSS2) and TMPRSS11D are human proteases that enable SARS-CoV-2 and Influenza A/B virus infections, but their biochemical mechanisms for facilitating viral cell entry remain unclear. We show these proteases spontaneously and efficiently cleave their own zymogen activation motifs, activating their broader protease activity on cellular substrates. We determine TMPRSS11D co-crystal structures with a native and an engineered activation motif, revealing insights into its autocleavage activation and distinct substrate binding cleft features. Leveraging this structural data, we develop nanomolar potency peptidomimetic inhibitors of TMPRSS11D and TMPRSS2. We show that a broad serine protease inhibitor that underwent clinical trials for TMPRSS2-targeted COVID-19 therapy, nafamostat mesylate, was rapidly cleaved by TMPRSS11D and converted to low activity derivatives. In this work, we develop mechanistic insights into human protease viral tropism and highlight both the strengths and limitations of existing human serine protease inhibitors, informing future drug discovery efforts targeting these proteases.

PubMed: 40348740
DOI: 10.1038/s41467-025-59677-3

Experimental method

X-RAY DIFFRACTION (1.59 Å)